Search Results

Overview

Uniprot IDF1S4P6
Protein NameEukaryotic translation initiation factor 3 subunit A
Gene NameEIF3A
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
100 YLKLAEEKTEAAKEE
1363 RESLRRTKNETDEDG
196 TRKAEFRKLCDNLRM
308 HLSREMRKNLTQEEM
409 KLCERVTKVLNWIRE
538 AKALEVIKPAHILQE
603 QREAELQKVRKAEEE
68 LRKSHLAKEGLYQYK
694 RLKNQEKKIDYFERA
777 SVYEEKLKQFEERLA
95 DVVRAYLKLAEEKTE

Function

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression

Protein Sequence

10 MPAYFQRPEN 20 ALKRANEFLE 30 VGKKQPALDV 40 LYDVMKSKKH 50 RTWQKIHEPI 60 MLKYLELCVD 70 LRKSHLAKEG 80 LYQYKNICQQ 90 VNIKSLEDVV 100 RAYLKLAEEK 110 TEAAKEESQQ 120 MVLDIEDLDN 130 IQTPESVLLS 140 AVSGEDTQDR 150 TDRLLLTPWV 160 KFLWESYRQC 170 LDLLRNNSRV 180 ERLYHDIAQQ 190 AFKFCLQYTR 200 KAEFRKLCDN 210 LRMHLSQIQR 220 HHNQSTAINL 230 NNPESQSMHL 240 ETRLVQLDSA 250 ISMELWQEAF 260 KAVEDIHGLF 270 SLSKKPPKPQ 280 LMANYYNKVS 290 TVFWKSGNAL 300 FHASTLHRLY 310 HLSREMRKNL 320 TQEEMQRMST 330 RVLLATLSIP 340 ITPERTDIAR 350 LLDMDGIIVE 360 KQRRLATLLG 370 LQAPPTRIGL 380 INDMVRFNVL 390 QYVVPEVKDL 400 YNWLEVEFNP 410 LKLCERVTKV 420 LNWIREQPEK 430 EPELQQYVPQ 440 LQNNTILRLL 450 QQVAQIYQSI 460 EFSRLTSLVP 470 FVDAFQLERA 480 IVDAARHCDL 490 QVRIDHTSRT 500 LSFGSDLNYA 510 TREDAPIGPH 520 LQSMPSEQIR 530 NQLTAMSSVL 540 AKALEVIKPA 550 HILQEKEEQH 560 QLAVTAYLKN 570 SRKEHQRILA 580 RRQTIEERKE 590 RLESLNIQRE 600 KEELEQREAE 610 LQKVRKAEEE 620 RLRQEAKERE 630 KERILQEHEQ 640 IKKKTVRERL 650 EQIKKTELGA 660 KAFKDIDIED 670 LEELDPDFIM 680 AKQVEQLEKE 690 KKELQERLKN 700 QEKKIDYFER 710 AKRLEEIPLI 720 KSAYEEQRIK 730 DMDLWEQQEE 740 ERITTMQLER 750 EKALEHKNRM 760 SRMLEDRDLF 770 VMRLKAARQS 780 VYEEKLKQFE 790 ERLAEERHNR 800 LEERKRQRKE 810 ERRITYYREK 820 EEEEQRRAEE 830 QMLKEREERE 840 RAERAKREEE 850 LREYQERVKK 860 LEEVERKKRQ 870 RELEIEERER 880 RREEERRLGE 890 DPLSRKDSRW 900 GDRDSEGTWR 910 KGPEIDSEWR 920 RGPPEKDWRR 930 GEGRDEERPH 940 RRDDDRPRRL 950 GDDEERESSL 960 RPDEDRGPRR 970 GMDDDRGPRR 980 GLDEDRFSRR 990 GADDDRPSWR 1000 NTDDDRPPRR 1010 IGDEDRGSWR 1020 HADDDRPPRR 1030 GLDEDRGSWR 1040 TADEDRGPRR 1050 GMDEDRGPRR 1060 GGVDDERSSW 1070 RNADDDRPRR 1080 GMDDDRGPRR 1090 GMDDDRGPRR 1100 GMDDDRGPRR 1110 GLDDDRGPWR 1120 NTDDDRISRR 1130 GADDDRGPWR 1140 NMDDDRIPRR 1150 GDDDRIPRRG 1160 DDSRPGPWRP 1170 FVKPGGWREK 1180 ERAREESWGP 1190 PRESRPSEDR 1200 EWDREKERDR 1210 DNQDRDENER 1220 EPERDRDRER 1230 DRERDRDRDG 1240 DREDRFRRPR 1250 DEGGWRRGPA 1260 EESSSWRDAS 1270 RRDDRDRDDR 1280 RRDDRRDLRE 1290 RRDDRDRRGP 1300 PLRSEREEVS 1310 SWRRADDRKD 1320 DRAEERDAPR 1330 RVPPPALSRD 1340 RERDRDRDRE 1350 RDGEKEKTSW 1360 RADKDRESLR 1370 RTKNETDEDG WTTVRR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0016282 eukaryotic 43S preinitiation complex
Cellular Component GO:0033290 eukaryotic 48S preinitiation complex
Cellular Component GO:0071540 eukaryotic translation initiation factor 3 complex, eIF3e
Cellular Component GO:0071541 eukaryotic translation initiation factor 3 complex, eIF3m
Cellular Component GO:0043614 multi-eIF complex
Cellular Component GO:0014069 postsynaptic density
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0003743 translation initiation factor activity
Biological Process GO:0001732 formation of cytoplasmic translation initiation complex
Biological Process GO:0075522 IRES-dependent viral translational initiation
Biological Process GO:0002188 translation reinitiation
Biological Process GO:0075525 viral translational termination-reinitiation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.