Overview
| Uniprot ID | F1S586 |
|---|
| Protein Name | Queuine tRNA-ribosyltransferase catalytic subunit 1 |
|---|
| Gene Name | QTRT1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 367 |
VRTSIVEKRFPDFVR |
Function
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product
Protein Sequence
10
MAAAASQTSL
20
DSAPRILRLV
30
AECSRSRARA
40
GELRLPHGPV
50
ATPVFMPVGT
60
QATMKGITAE
70
QLDALGCRIC
80
LGNTYHLGLR
90
PGPDLIQKAH
100
GLHGFMNWPH
110
NLLTDSGGFQ
120
MVSLVSLSEV
130
TEEGVRFRSP
140
YDGDETLLSP
150
ERSVEIQNAL
160
GSDIIMQLDD
170
VVSSTVTGPR
180
VEEAMYRSIR
190
WLDRCIAAHR
200
WPDKQNLFAI
210
IQGGLDADLR
220
TTCLEEMTKR
230
DVPGFAIGGL
240
SGGESKSQFW
250
RMVALSTSRL
260
PKDKPRYLMG
270
VGYATDLVVC
280
VALGCDMFDC
290
VFPTRTARFG
300
SALVPTGNLQ
310
LKKKQYEKDF
320
RPIDPECACP
330
TCQKHSRAFL
340
HTLLHSDNTA
350
ALHHLTVHNI
360
AYQLRLMSAV
370
RTSIVEKRFP
380
DFVRDFMRTM
390
YGDPTLCPTW
400
ATEALASVGI
TLS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0032473 |
cytoplasmic side of mitochondrial outer membrane |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0120507 |
tRNA-guanine transglycosylase complex |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0008479 |
tRNA-guanosine(34) queuine transglycosylase activity |
| Biological Process |
GO:0002099 |
tRNA wobble guanine modification |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.