Overview
| Uniprot ID | F1S8D4 |
|---|
| Protein Name | Methylosome protein WDR77 |
|---|
| Gene Name | WDR77 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 201 |
LWDTRCPKPASQIGY |
| 3 |
*****MRKETPPSLV |
Function
Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage
Protein Sequence
10
MRKETPPSLV
20
PPAAREWNLP
30
PNAPACMERQ
40
LEAARYRSDG
50
ALLLGASSLS
60
GRCWAGSLWL
70
FKDPCAAPNE
80
GFCSAGVQTE
90
AGVADLTWVG
100
DRGILVASDS
110
GAVELWELDE
120
NETLIVSKFC
130
KYEHDDIVSS
140
VSVLSSGTQA
150
VSGSKDFCIK
160
VWDLAQQMVL
170
NSYRAHSGQV
180
TCVAASPHKD
190
SVFLSCSEDN
200
RILLWDTRCP
210
KPASQIGYRA
220
SGYLPTSLAW
230
HPQRSEVFVF
240
GDENGTVSLV
250
DTKSASCALS
260
ATVHSQSVTG
270
LVFSPHSTPF
280
LASVSEDCSL
290
AVLDSGLSKV
300
FRSRAHRDFV
310
RDATWSPLNH
320
SLLTTVGWDH
330
QVVHHILPTE
340
PFPEPGLKSA
AE
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0031465 |
Cul4B-RING E3 ubiquitin ligase complex |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005794 |
Golgi apparatus |
| Cellular Component |
GO:0034709 |
methylosome |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Molecular Function |
GO:0008327 |
methyl-CpG binding |
| Molecular Function |
GO:0003713 |
transcription coactivator activity |
| Molecular Function |
GO:1990756 |
ubiquitin-like ligase-substrate adaptor activity |
| Biological Process |
GO:0000209 |
protein polyubiquitination |
| Biological Process |
GO:0006511 |
ubiquitin-dependent protein catabolic process |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.