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Overview

Uniprot IDF1SA74
Protein NameC-1-tetrahydrofolate synthase, cytoplasmic
Gene NameMTHFD1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
262 DVAYSEAKERAGFIT
354 LYGETKAKVLLSSLE
488 PSVNGVRKFSDIQLR
543 TNDRFLRKITIGQAP
553 IGQAPTEKGHSRTAQ
61 NVKLKAAKEIGIKAM
684 MEKFFNIKCRYSGLR
775 DLVSRLAKEHGAFDA
878 LSHNPEQKGVPTGFV

Function

Trifunctional enzyme that catalyzes the interconversion of three forms of one-carbon-substituted tetrahydrofolate: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate, 5,10-methenyltetrahydrofolate and (6S)-10-formyltetrahydrofolate. These derivatives of tetrahydrofolate are differentially required in nucleotide and amino acid biosynthesis, (6S)-10-formyltetrahydrofolate being required for purine biosynthesis while (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate is used for serine and methionine biosynthesis for instance

Protein Sequence

10 MAPAEILNGK 20 VVSAQIREKL 30 KSKVAQMKEQ 40 VPGFTPGLAI 50 LQVGNRDDSN 60 LYINVKLKAA 70 KEIGIKAMHI 80 KLPRTATESE 90 VLKCITSLNE 100 DLTVHGFILQ 110 LPLDSENPIN 120 AEALINAIAP 130 EKDVDGLTSI 140 NAGKLARGDL 150 NDCFIPCTPK 160 GCLELIKQTG 170 VQIAGRQAVV 180 IGRSKIVGAP 190 MHDLLLWNHA 200 TVTTCHSKTA 210 NLNEEVNKGD 220 IVVVAAGKPE 230 MVKGEWIKPG 240 AVVIDCGINC 250 VPDDTKPSGK 260 RIVGDVAYSE 270 AKERAGFITP 280 VPGGVGPMTV 290 AMLMQSTVES 300 AEHFLEKFRP 310 GKWIIQYNKL 320 NLKTPVPSDI 330 DISRSCKPKP 340 IGNLAREIGL 350 LSEEVELYGE 360 TKAKVLLSSL 370 ERLKHQPDGK 380 YVVVTGITPT 390 PLGEGKSTTT 400 IGLVQALGAH 410 LHQNVFACVR 420 QPSQGPTFGI 430 KGGAAGGGYS 440 QVIPMEEFNL 450 HLTGDIHAIT 460 AANNLVAAAI 470 DARMFHEQTQ 480 TDKALFNRLV 490 PSVNGVRKFS 500 DIQLRRLWRL 510 GIEKTDPTTL 520 TDEEINRFAR 530 LDIDPETITW 540 QRVLDTNDRF 550 LRKITIGQAP 560 TEKGHSRTAQ 570 FDISVASEIM 580 AVLALTSSLE 590 DMRERLSKMV 600 VASSKKGEPI 610 SAEDLGVSGA 620 LTVLMKDAIK 630 PNLMQTLEGT 640 PVFVHAGPFA 650 NIAHGNSSII 660 ADRIALKLVG 670 PEGFVVTEAG 680 FGADIGMEKF 690 FNIKCRYSGL 700 RPHVVVLVAT 710 VRALKMHGGG 720 PTVTAGLPLP 730 KAYIEENLEL 740 VEKGFSNLRK 750 QIENAKMFGV 760 PVVVAVNAFK 770 TDTEAELDLV 780 SRLAKEHGAF 790 DAVKCTHWAE 800 GGKGALALAQ 810 AVQRAAQAPS 820 SFQLLYDLEL 830 PIEDKIRIIA 840 QKIYGADDIE 850 LLPEAQHKAE 860 VYTKQGFGNL 870 PICMAKTHLS 880 LSHNPEQKGV 890 PTGFVLPIRD 900 IRASVGAGFL 910 YPLVGTMSTM 920 PGLPTRPCFY 930 DIDLDPETEQ VNGLF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004329 formate-tetrahydrofolate ligase activity
Molecular Function GO:0004477 methenyltetrahydrofolate cyclohydrolase activity
Molecular Function GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity
Biological Process GO:0009257 10-formyltetrahydrofolate biosynthetic process
Biological Process GO:0046655 folic acid metabolic process
Biological Process GO:0035999 tetrahydrofolate interconversion

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.