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Overview

Uniprot IDF1SB38
Protein NamePseudouridylate synthase 7 homolog
Gene NamePUS7
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
417 REEWAKTKDPAAALR
42 VSECCLTKGQDGLEN
530 DIIYPKHKISEAYRE

Function

Pseudouridylate synthase that catalyzes pseudouridylation of RNAs. Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions. Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing. In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types

Protein Sequence

10 MEMTEMTGVS 20 LKRGSIAVED 30 NDIAAPVEEA 40 KRQKVSECCL 50 TKGQDGLENN 60 SLPSSEKMPG 70 SPEVAGAGED 80 KKNSDVQTEG 90 EEEEEAESFA 100 DMMKHGLTEL 110 DVGITKFVSS 120 HQGFSGILKE 130 RYSDFIVHEI 140 GKDGRISHLN 150 DLSVPVDKED 160 PSKDVLTLLT 170 DEEKQRLEEL 180 QLFKNKETSV 190 AIEVIEDTKE 200 KRTIIHQGIK 210 SLFPGLETKT 220 EDREGKKYIV 230 AYHAAGKKAL 240 ANPRKHSWPK 250 SRGSYCHFVL 260 YKENKDTMDA 270 INLLSKYLRV 280 KPNIFSYMGT 290 KDKRAITVQE 300 IAVLKITAQR 310 LAHLNKCLMN 320 FKLGNFSYQK 330 KPLKLGELQG 340 NHFTVVLRNI 350 TGTDDQVQQA 360 MNSLKEIGFI 370 NYYGMQRFGT 380 TAVPTYQVGR 390 AILQNSWTEV 400 MDLILKPRSG 410 AEKGYLVKCR 420 EEWAKTKDPA 430 AALRKLPVKR 440 CVEGQLLRGL 450 SKYGMKNIVS 460 AFGIIPRNNR 470 LMYIHSYQSY 480 VWNNMVSKRI 490 EEYGLKPVPG 500 DLVLKGATAT 510 YIEEEDVNNY 520 SIHDVVMPLP 530 GFDIIYPKHK 540 ISEAYREMLT 550 ADNLDIDNMR 560 HKIRDYSLSG 570 AYRKIIIRPQ 580 NVSWEVVAYD 590 DPKIPLFNTD 600 VDNLEGKPPP 610 VFASEGKYRA 620 LKMDFSLPPS 630 TYATMAIREV 640 LKMDTSIKKQ TQLNTTWLR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005634 nucleus
Molecular Function GO:0019899 enzyme binding
Molecular Function GO:0009982 pseudouridine synthase activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0160150 tRNA pseudouridine(13) synthase activity
Biological Process GO:0006397 mRNA processing
Biological Process GO:1990481 mRNA pseudouridine synthesis
Biological Process GO:0017148 negative regulation of translation
Biological Process GO:0001522 pseudouridine synthesis
Biological Process GO:1902036 regulation of hematopoietic stem cell differentiation
Biological Process GO:2000380 regulation of mesoderm development
Biological Process GO:0008380 RNA splicing
Biological Process GO:0031119 tRNA pseudouridine synthesis

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.