Overview
| Uniprot ID | F1SDN2 |
|---|
| Protein Name | Trifunctional enzyme subunit beta, mitochondrial |
|---|
| Gene Name | HADHB |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 238 |
QRLSLISKFRLNFLS |
| 445 |
NYMGRKAKVGSPPLE |
Function
Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity
Protein Sequence
10
MTSLLTYTFK
20
SLPSTSKWAL
30
RFCKLYLFFL
40
FKISFGYVSV
50
LHRHGIVLLF
60
VSTLFCLPAL
70
RPLSSSSQLQ
80
AAAAVQTKSK
90
KTLAKPNIRN
100
IVVVDGVRIP
110
FLQSGTSYKE
120
LMPHDLARAA
130
LLGLLHRTSV
140
PKDVVDYLVF
150
GTVIQEVKTS
160
NVAREAALGA
170
GFSDKTPAHT
180
VTMACISSNQ
190
AMTTGVSLIA
200
SGQFDVVVAG
210
GVELMSDIPI
220
RHSRKMRKMM
230
LDLNKAKTLG
240
QRLSLISKFR
250
LNFLSPELPA
260
VAEFSTSETM
270
GHSADRLAAA
280
FAVSRSEQDE
290
YALRSHSLAK
300
KAQDEGLLSD
310
VVPFKVPGKD
320
TVTQDNGIRP
330
SSLEQMAKLK
340
PAFIKPYGTV
350
TAANSSFLTD
360
GASAVLIMAE
370
EKALAMGYKP
380
KAYLRDFTYV
390
SQDPKDQLLL
400
GPTYATPKVL
410
EKAGLTMNDI
420
DAFEFHEAFS
430
GQILANLKAM
440
DSDWFAQNYM
450
GRKAKVGSPP
460
LEKFNNWGGS
470
LSLGHPFGAT
480
GCRLVMTAAN
490
RLRKEGGQYG
500
LVAACAAGGQ
510
GHAMIVEAYP
K
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0016507 |
mitochondrial fatty acid beta-oxidation multienzyme complex |
| Cellular Component |
GO:0005743 |
mitochondrial inner membrane |
| Cellular Component |
GO:0042645 |
mitochondrial nucleoid |
| Cellular Component |
GO:0005741 |
mitochondrial outer membrane |
| Molecular Function |
GO:0003985 |
acetyl-CoA C-acetyltransferase activity |
| Molecular Function |
GO:0050633 |
acetyl-CoA C-myristoyltransferase activity |
| Molecular Function |
GO:0106222 |
lncRNA binding |
| Biological Process |
GO:0071222 |
cellular response to lipopolysaccharide |
| Biological Process |
GO:0006635 |
fatty acid beta-oxidation |
| Biological Process |
GO:0010467 |
gene expression |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.