Search Results

Overview

Uniprot ID	F1SHX3
Protein Name	Telomere-associated protein RIF1
Gene Name	RIF1
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
1009	DSFLAQTKDAKESMK
1022	MKPVAKSKLESSTPK
1726	GCDCCGEKSQPQEKS
776	IKYQPKIKSPQRPSD
786	QRPSDWSKKKKEPLG

Function

Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs. In response to DNA damage, interacts with ATM-phosphorylated TP53BP1. Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs. Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs. In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase. Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Promotes NHEJ of dysfunctional telomeres

Protein Sequence

10 MTAPGPSHLA 20 PLLETLEDPS 30 ASQGEQTDAY 40 LTLTSRMTGE 50 EGKEIIIEIE 60 KKLPRLFKVL 70 KNHITSQNSE 80 LSSAALQALG 90 FCLYNPKITS 100 ALSETIIQEL 110 LSKLNDTIKS 120 SDKNVRTRAL 130 WVISKQTFPP 140 EVLGKVVSSI 150 IDSLEIVYNR 160 GEMHSAVVDY 170 EALNVIIRLI 180 EQAPIQMGEE 190 SIRWAKVVIP 200 LVVHSAQKVH 210 LRGATALEMG 220 MPLLLQKQQE 230 IASITEQLMT 240 TKLLSELQKL 250 FMSKNETYVL 260 KLWPLFVKLL 270 GKTLHRSGSF 280 INSLLQLEEL 290 GFRSGAPMIK 300 KIAFIAWKSL 310 IDNFALNPEI 320 LCSAKRLKLL 330 MQPLSSIHVR 340 TEALALTKLE 350 VWWYLLMRLG 360 PHLPANFEQV 370 CVPLIQSTIS 380 IDSNASPQGS 390 SSRVATSPGL 400 NPMTPVHKGA 410 SPVTPRMNLS 420 SNFGGMATIP 430 SIQLLGLEML 440 LHFLLGPEAL 450 SFAKQNKLIL 460 SLEPLEHPLI 470 SSSSFFSKHA 480 NTLITAVHDS 490 FVAVGKDASD 500 AVIGAIWKEL 510 ISLVKSVVES 520 GNRKERPGSE 530 VLTLLLKCLE 540 SIVKSEVFPV 550 SKMLVLMEIT 560 IKGLPQKVLG 570 SPAYQVANMD 580 ILNGTPALFL 590 IQLIFNNNLE 600 CGVEDERFFL 610 SLETLVGCVL 620 SGPTSPLAFS 630 DSVLNVINQN 640 AKQLENKEHL 650 WRMWSIIVTP 660 LTELINQTNE 670 VNQGDALEHN 680 FSAIYGALTL 690 PINHIFSAQK 700 IPVATMKTLL 710 KTWSELYRAF 720 ARCAALVATA 730 EENLCCEELS 740 SKIMSSLEDE 750 GLSNLLFLDR 760 IIHIVTVMVD 770 CIDFSPYNIK 780 YQPKIKSPQR 790 PSDWSKKKKE 800 PLGKLASLFK 810 LIVRVIYSFH 820 TLSLKEVHCD 830 TLLIIGNSII 840 SILSNVLGHI 850 SLPSMIRKIF 860 ATLTKPLVLF 870 YENSKFDEVP 880 KVYTCMNTKL 890 EKLLGEIIAC 900 LHISYTGTYD 910 SELLEQISPL 920 LCVIFSHKNK 930 QIRKQSAQFW 940 NATFAKVAML 950 IYPEELKPVL 960 RQAKQKSLLL 970 LPGLENVEIM 980 EESSGPYSDT 990 TENSQLNMKI 1000 SGMERKSSGK 1010 RDSFLAQTKD 1020 AKESMKPVAK 1030 SKLESSTPKS 1040 KSEMLLEEEM 1050 STDFVFIPPE 1060 GKETKERILT 1070 EHQKEVLKAK 1080 RCDIPAMYNN 1090 LDVSQDTLFS 1100 QYSQEESMEI 1110 PTLTEKSKED 1120 SKMIFKEEQK 1130 ESDTVIPQLV 1140 VENRGMDEHL 1150 EKATFSNNEN 1160 GSVEETSSEI 1170 LSSNEDARGK 1180 TLVIRKKTTT 1190 ECASSTENTV 1200 VVSSNSVSNV 1210 TIPGNPPQPT 1220 SRRQSFITLE 1230 KFDGSENRPF 1240 SPSPLNNMSS 1250 TVTVKNSQEG 1260 TTKTDNPSKA 1270 KKVVLLQSDS 1280 EKIVHGIKRS 1290 GRKSSRSEPT 1300 GNKKSKPLMR 1310 SDQEKSTRES 1320 FDGMVALENN 1330 PPGLLNETEC 1340 VLDHQVHLSE 1350 SAVEYGDTTL 1360 KPTVENAVLL 1370 ESSTVEDKSL 1380 EINLESKENT 1390 PPAITTADQT 1400 VNEDSHVQVT 1410 SNQKTLRRSL 1420 RRRSETTEPA 1430 TDSQDKENNH 1440 QKRERRKEEE 1450 KTLQKSPLHV 1460 KDDVLPKQKL 1470 MSEQTVQENL 1480 IEKGSNLHEK 1490 TLGETSADVE 1500 IDENRRKPDL 1510 EDTKSEGGGT 1520 QDVTDKSSEK 1530 PVRGRTRYQT 1540 RRASQGLLSS 1550 IENSESDSSE 1560 AKEEGSKKKR 1570 SGKWKNKSSD 1580 SVDIEDQEEK 1590 MVRQESIKVE 1600 NQTHDYEMNS 1610 EVDKDIKSQI 1620 CEKDDIDIIT 1630 LQDSTVSSDL 1640 FQVSDDVSNE 1650 GKSKTYRCAE 1660 HSFSNPPVPE 1670 SNLRTRNANK 1680 RLQKRDSIEN 1690 SVGESSKTGT 1700 SDISLLSEKT 1710 LQTLECQHKR 1720 SRRVRRSKGC 1730 DCCGEKSQPQ 1740 EKSFIGLKNT 1750 ENFDVKVSET 1760 KADVQTPVTI 1770 SEPSKADLHS 1780 EVKLSDEHHS 1790 VNFHLDLKEE 1800 NDTINDSSVI 1810 SESEFKENIQ 1820 DSFPSEMVNL 1830 NEETCDRTTE 1840 EAVSLESQEP 1850 SNKRCKTLGP 1860 CLDDSKDTSL 1870 ECFTLETKEE 1880 KPAEATPEKE 1890 LSVENIVNAE 1900 ENECKVKELN 1910 QEVDDAMELE 1920 VESDKSEVSF 1930 FEQKSDISKE 1940 GLMEEISEGK 1950 DTSEAHVAEE 1960 MNADEAVTEE 1970 FNSDVGHSDS 1980 TTSVKVRTQM 1990 EFSEQIAVGK 2000 LDRGSDESDP 2010 GSSEEMNAET 2020 ENCAETVIGE 2030 VTAETDHISE 2040 TEDEDFTSKA 2050 SKPVEAETKG 2060 LNVEMDSFVP 2070 DTLEMSTEEG 2080 KVDPRKTETN 2090 VELNKFDKTE 2100 LDDNHMVVRN 2110 DGILQEVHHT 2120 SEKVEETPQS 2130 LTSETAVSEL 2140 IPEDNNTSPQ 2150 KLKDLDPLLM 2160 STNDSPSGMQ 2170 TRCVWSPLAS 2180 PSTSILKRGL 2190 KRPQEDEISS 2200 PVHKVRRVSF 2210 ADPIYQAGLA 2220 DDIDRRCSII 2230 RCHSSNSSSM 2240 VKSAKTSPTA 2250 HSKHNTTSTK 2260 GFLSPGSRSP 2270 KFKSSKKCLI 2280 AEMAKESVPC 2290 PTESVYPALV 2300 NCAAPVDIIL 2310 PQITSNMWAR 2320 GLGQLIRAKN 2330 IKTIGDLSTL 2340 TASEIKTLPI 2350 RSPKVSNVKK 2360 ALRVYHEQQV 2370 KSRGLEEIPV 2380 FDISEKTING 2390 MENKSVSPDE 2400 ERLASDLIDP 2410 VALETPLSKN 2420 LVAQISALAL 2430 QLDSEDLHNY 2440 SGSQLFEMHE 2450 KLGSMANCII 2460 KNLQSRWRSP AHENSI

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0000785	chromatin
Biological Process	GO:0000122	negative regulation of transcription by RNA polymerase II
Biological Process	GO:2001034	positive regulation of double-strand break repair via nonhomologous end joining
Biological Process	GO:0045830	positive regulation of isotype switching
Biological Process	GO:0031509	subtelomeric heterochromatin formation
Biological Process	GO:0000723	telomere maintenance
Biological Process	GO:0043247	telomere maintenance in response to DNA damage
Cellular Component	GO:0140445	chromosome, telomeric repeat region
Cellular Component	GO:0000793	condensed chromosome
Cellular Component	GO:0001939	female pronucleus
Cellular Component	GO:0001940	male pronucleus
Cellular Component	GO:0031965	nuclear membrane
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0035861	site of double-strand break
Cellular Component	GO:0051233	spindle midzone
Biological Process	GO:0006281	DNA repair
Biological Process	GO:2000042	negative regulation of double-strand break repair via homologous recombination

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.