Search Results

Overview

Uniprot IDF1SII4
Protein NameGlycine--tRNA ligase
Gene NameGARS1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
102 DKAVAELKARKRVLE
111 RKRVLEAKELALQPK
118 KELALQPKDDVVDRA
126 DDVVDRAKMEDTLKR
132 AKMEDTLKRRFFYDQ
200 KTSGHVDKFADFMVK
207 KFADFMVKDVKNGEC
210 DFMVKDVKNGECFRA
222 FRADHLLKAHLQKLM
227 LLKAHLQKLMSDKKC
429 KVGVSPDKLRFRQHM
480 SCHARATKVPLVAEK
487 KVPLVAEKPLKEPIS
512 PNKGAIGKAYKKDAK
543 MEKLLNEKGEFTVET
553 FTVETEGKTFQLTKD
559 GKTFQLTKDMVNVKR
565 TKDMVNVKRFQKTLH
652 TRHGVSHKVDDSSGS
88 VRKLKEDKAPQVDVD
96 APQVDVDKAVAELKA

Function

Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis

Protein Sequence

10 MPSLRPALLR 20 AARAALLLSP 30 PPRLPAQPPL 40 LPCRHISKPA 50 RAPISSPAAS 60 AAASRSSMDG 70 VGTEEVLAPL 80 RLAVRQQGDL 90 VRKLKEDKAP 100 QVDVDKAVAE 110 LKARKRVLEA 120 KELALQPKDD 130 VVDRAKMEDT 140 LKRRFFYDQA 150 FAIYGGVSGL 160 YDFGPVGCAL 170 KNNIIQTWRQ 180 HFIQEEQILE 190 IDCTMLTPEP 200 VLKTSGHVDK 210 FADFMVKDVK 220 NGECFRADHL 230 LKAHLQKLMS 240 DKKCSAEKKS 250 EMESVLAQLE 260 NYGQQELGDL 270 FVNYNVKSPI 280 TGNDLSPPVS 290 FNLMFKTFIG 300 PGGNMPGYLR 310 PETAQGIFLN 320 FKRLLEFNQG 330 KLPFAAAQIG 340 NSFRNEISPR 350 SGLIRVREFT 360 MAEIEHFVDP 370 SEKDHPKFQN 380 VADLHLHLYS 390 AKAQVSGQSA 400 RKMRLGDAVE 410 QGVINNSVLG 420 YFIGRIYLYL 430 VKVGVSPDKL 440 RFRQHMENEM 450 AHYACDCWDA 460 ESKTSYGWIE 470 IVGCADRSCY 480 DLSCHARATK 490 VPLVAEKPLK 500 EPISFLTVNV 510 VQFEPNKGAI 520 GKAYKKDAKL 530 VMEYLAICDE 540 CYITEMEKLL 550 NEKGEFTVET 560 EGKTFQLTKD 570 MVNVKRFQKT 580 LHVEEVVPSV 590 IEPSFGLGRI 600 MYTVFEHTFH 610 VREGDEQRTF 620 FSFPAVVAPF 630 KCSVLPLSQN 640 QEFMPFVKEL 650 SEALTRHGVS 660 HKVDDSSGSI 670 GRRYARTDEI 680 GVAFGITIDF 690 DTVNKTPHTA 700 TLRDRDSMRQ 710 IRAEVSELPS 720 VVRDLANGSI 730 TWADVEARYP 740 LFEGQETGKK ETIEE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0030424 axon
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0030141 secretory granule
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004081 bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity
Molecular Function GO:0004820 glycine-tRNA ligase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0046983 protein dimerization activity
Molecular Function GO:0016740 transferase activity
Biological Process GO:0015966 diadenosine tetraphosphate biosynthetic process
Biological Process GO:0070150 mitochondrial glycyl-tRNA aminoacylation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.