Overview
| Uniprot ID | F1SIJ9 |
|---|
| Protein Name | Phosphoserine aminotransferase |
|---|
| Gene Name | PSAT1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 124 |
KAAEEAKKFGTVNIV |
| 134 |
TVNIVHPKLGSYTKI |
| 317 |
PFRIGNTKGDDALEK |
| 324 |
KGDDALEKRFLDKAL |
| 329 |
LEKRFLDKALELKMI |
| 334 |
LDKALELKMISLKGH |
Function
Involved in L-serine biosynthesis via the phosphorylated pathway, a three-step pathway converting the glycolytic intermediate 3-phospho-D-glycerate into L-serine. Catalyzes the second step, that is the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and alpha-ketoglutarate. Acts as an inhibitor of ferroptosis in response to interferon-gamma (IFNG) by promoting GPX4 stability: following phosphorylation by CAMK2A, PSAT1 interacts with GPX4 and provides 2-oxoglutarate to EGLN3, leading to GPX4 hydroxylation and stability
Protein Sequence
10
MRHSCFYNYL
20
LLNRVEISLG
30
QSLLCVVVLL
40
EIQKELLDYK
50
GVGISVLEMS
60
HRSSDFSKII
70
NNTENLVREL
80
LSIPDNYKVI
90
FVQGGGCGQF
100
SAVPLNLIGL
110
KAGRCADYVV
120
TGSWSAKAAE
130
EAKKFGTVNI
140
VHPKLGSYTK
150
IPDPSTWNLS
160
PDASYVYYCA
170
NETVHGVEFD
180
FIPDVKGAVL
190
VSDMSSNFLS
200
KPVDVSKFGV
210
IFAGAQKNVG
220
SAGVTVVIVR
230
DDLLGFALRE
240
CPSILDYKVQ
250
AGNNSLYNTP
260
PCFSVYVMGL
270
VLEWIKNNGG
280
AAAMEKLSCI
290
KSQMIYDIID
300
NSQGFYMSSG
310
APDRSKMNIP
320
FRIGNTKGDD
330
ALEKRFLDKA
340
LELKMISLKG
350
HRSVGGIRAS
360
LYNAVTIEDV
370
QKLAAFMKNF
LEMHQL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Molecular Function |
GO:0004648 |
O-phospho-L-serine:2-oxoglutarate aminotransferase activity |
| Molecular Function |
GO:0030170 |
pyridoxal phosphate binding |
| Biological Process |
GO:0006564 |
L-serine biosynthetic process |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.