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Overview

Uniprot IDF1SJL5
Protein NameATP-dependent clpX-like chaperone, mitochondrial
Gene NameCLPX
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
171 KPPPPPKKIYNYLDK

Function

ATP-dependent chaperone that functions as an unfoldase. As part of the ClpXP protease complex, it recognizes specific protein substrates, unfolds them using energy derived from ATP hydrolysis, and then translocates them to the proteolytic subunit (CLPP) of the ClpXP complex for degradation. Thanks to its chaperone activity, it also functions in the incorporation of the pyridoxal phosphate cofactor into 5-aminolevulinate synthase, thereby activating 5-aminolevulinate (ALA) synthesis, the first step in heme biosynthesis. This chaperone is also involved in the control of mtDNA nucleoid distribution, by regulating mitochondrial transcription factor A (TFAM) activity

Protein Sequence

10 MSGCGACTCG 20 ASAARLITSS 30 LASAQRGISC 40 GRIHIPVLGR 50 LGTFETQLLR 60 RVPFRTFTET 70 PAYFASKDGI 80 SKDGSGDGNK 90 KSASEGSSKK 100 SGSGNSGKGG 110 NQLRCPKCGD 120 LCTHVETFVS 130 STRFVKCEKC 140 HHFFVVLSEA 150 DSKKSIIKEP 160 ESAAEAVKLA 170 FQQKPPPPPK 180 KIYNYLDKYV 190 VGQSFAKKVL 200 SVAVYNHYKR 210 IYNNIPANLR 220 QQAEVEKQTS 230 LTPRELLQIA 240 GISPHGNALG 250 ASMQQQVNQQ 260 IPQEKRGGEV 270 LDSSHDDIKL 280 EKSNILLLGP 290 TGSGKTLLAQ 300 TLAKCLDVPF 310 AICDCTTLTQ 320 AGYVGEDIES 330 VIAKLLQDAN 340 YNVEKAQQGI 350 VFLDEVDKIG 360 SVPGIHQLRD 370 VGGEGVQQGL 380 LKLLEGTIVN 390 VPEKNSRKLR 400 GETVQVDTTN 410 ILFVASGAFN 420 GLDRIISRRK 430 NEKYLGFGTP 440 SNLGKGRRAA 450 AAADLANRSG 460 ESNTHQDIEE 470 KDRLLRHVEA 480 RDLIEFGMIP 490 EFVGRLPVVV 500 PLHSLDEKTL 510 VQILTEPRNA 520 VIPQYQALFS 530 MDKCELNVTE 540 DALKAIARLA 550 LERKTGARGL 560 RSIMEKLLLE 570 PMFEVPNSDI 580 VCVEVDKEVV 590 EGKKEPGYIR 600 APTKESSEEE 610 YDSGVEEEGW PRQADAANS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0042645 mitochondrial nucleoid
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0140662 ATP-dependent protein folding chaperone
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0008233 peptidase activity
Molecular Function GO:0051082 unfolded protein binding
Biological Process GO:0006508 proteolysis

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.