Overview
| Uniprot ID | F1SN05 |
|---|
| Protein Name | Eukaryotic translation initiation factor 3 subunit J |
|---|
| Gene Name | EIF3J |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 156 |
FGKLLKDKITQYEKS |
| 200 |
LTVLCSEKQKQEKQS |
| 214 |
SKAKKKKKGVVPGGG |
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA
Protein Sequence
10
RREMAAAAAA
20
AAAGDSDSWD
30
ADTFSVDDPV
40
RKVGGGGTAG
50
GDRWEGEDED
60
EDVKVGAEVK
70
ISEKKKIAEK
80
IKEKERQQKK
90
RQEEIKKRLE
100
EPEEPKVLTP
110
EEQLADKLRL
120
KKLQEESDLE
130
LAKETFGVNN
140
TIYGIDAMNP
150
SSRDDFTEFG
160
KLLKDKITQY
170
EKSLYYASFL
180
EALVRDVCIS
190
LEIDDLKKIT
200
NSLTVLCSEK
210
QKQEKQSKAK
220
KKKKGVVPGG
230
GLKATMKDDL
240
ADYGGYDGGY
VQDYEDFM
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0016282 |
eukaryotic 43S preinitiation complex |
| Cellular Component |
GO:0033290 |
eukaryotic 48S preinitiation complex |
| Cellular Component |
GO:0005852 |
eukaryotic translation initiation factor 3 complex |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
| Biological Process |
GO:0001732 |
formation of cytoplasmic translation initiation complex |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.