Overview
| Uniprot ID | F1SNV5 |
|---|
| Protein Name | Lipid droplet-regulating VLDL assembly factor AUP1 |
|---|
| Gene Name | AUP1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 143 |
GELVESLKRFCASTR |
Function
Plays a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Plays a role in lipid droplet formation. Induces lipid droplet clustering. Recruits ubiquitin-conjugating enzyme UBE2G2 to lipid droplets which facilitates its interaction with ubiquitin ligases AMFR/gp78 and RNF139/TRC8, leading to sterol-induced ubiquitination of HMGCR and its subsequent proteasomal degradation. Also required for the degradation of INSIG1, SREBF1 and SREBF2. Plays a role in regulating assembly and secretion of very low density lipoprotein particles and stability of apolipoprotein APOB
Protein Sequence
10
MEPSPAPGPE
20
RLFDSHRLPG
30
DGFLLLALLL
40
YAPVGFCLLV
50
LRLFLGIHVF
60
LVSCALPDSV
70
LRRFVVRTMC
80
AVLGLVARQE
90
DSGLRDHRVR
100
VLISNHVTPF
110
DHNIVNLLTS
120
CSTPLLNSPP
130
SFVCWSRGFM
140
EMDGRGELVE
150
SLKRFCASTR
160
LPPTPLLLFP
170
EEEATNGREG
180
LLRFSSWPFS
190
IQDVVQPLTL
200
RVQRPLVSVT
210
VSDASWVSEL
220
LWSLFVPFTV
230
YQVRWLRPVH
240
RQLGEGNEEF
250
ALRVQQLLAK
260
ELGQTGTRLT
270
PADKAEHMKR
280
QRHPRLRPQS
290
AQSSFPPSPG
300
PSEAQLATLA
310
QRVKEVLPHV
320
PLGVIQRDLA
330
RTGCVDLTIT
340
NLLEGAVAFM
350
PEDITEGTQP
360
LPTASTPKFP
370
SSGPATPQPT
380
ALTFAKSSWA
390
RQESLQERKQ
400
ALYEYARRRF
TEKQAQEAD
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005776 |
autophagosome |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0005811 |
lipid droplet |
| Molecular Function |
GO:0043130 |
ubiquitin binding |
| Molecular Function |
GO:0031624 |
ubiquitin conjugating enzyme binding |
| Molecular Function |
GO:0031625 |
ubiquitin protein ligase binding |
| Biological Process |
GO:0036503 |
ERAD pathway |
| Biological Process |
GO:0140042 |
lipid droplet formation |
| Biological Process |
GO:0061724 |
lipophagy |
| Biological Process |
GO:1990044 |
protein localization to lipid droplet |
| Biological Process |
GO:0009615 |
response to virus |
| Biological Process |
GO:0030970 |
retrograde protein transport, ER to cytosol |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.