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Overview

Uniprot IDF1SPF3
Protein NameDNA replication licensing factor MCM2
Gene NameMCM2
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
217 GHGHNVFKERISDMC
385 RIQESPGKVAAGRLP
451 HVAKKDNKVAVGELT
493 IYGHEDIKRGLALAL
535 TAKSQFLKYIEKVSS
539 QFLKYIEKVSSRAIF

Function

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis

Protein Sequence

10 MAESSESFTV 20 ASSPAPRRRG 30 SDPLTSSPGR 40 SSRRTDALTS 50 SPGRDLPPFE 60 DESEGLLGTE 70 GPLEEEEEDG 80 EELIGDGMER 90 DYRAIPELDV 100 YEPEGLALDD 110 EDVEELTASQ 120 REAAERAMRQ 130 RDREAGRGLG 140 RMRRGLLYDS 150 DEEEEERPSR 160 KRRQVERATE 170 DGEEDEEMIE 180 SIENLEDLKG 190 HSVREWVSMA 200 GPRLEIHHRF 210 KNFLRTHVDG 220 HGHNVFKERI 230 SDMCKENRES 240 LVVNYEDLAA 250 REHVLAYFLP 260 EAPAELLQIF 270 DEAALEVVLA 280 MYPKYDRIAS 290 HIHVRISHLP 300 LVEELRSLRQ 310 LHLNQLIRTS 320 GVVTSCTGVL 330 PQLSMVKYNC 340 NKCGFVLGPF 350 CQSQNQEVKP 360 GSCPECQSAG 370 PFEVNMEETI 380 YQNYQRIRIQ 390 ESPGKVAAGR 400 LPRSKDAILL 410 ADLVDSCKPG 420 DEIELTGIYH 430 NNYDGALNTA 440 NGFPVFATVI 450 LANHVAKKDN 460 KVAVGELTDE 470 DVKMITSLSK 480 DQQIGEKIFA 490 SIAPSIYGHE 500 DIKRGLALAL 510 FGGEPKNPGG 520 KHKVRGDINV 530 LLCGDPGTAK 540 SQFLKYIEKV 550 SSRAIFTTGQ 560 GASAVGLTAY 570 VQRHPVSREW 580 TLEAGALVLA 590 DRGVCLIDEF 600 DKMNDQDRTS 610 IHEAMEQQSI 620 SISKAGIVTS 630 LQARCTVIAA 640 ANPIGGRYDP 650 SLTFSENVDL 660 TEPIISRFDI 670 LCVVRDTVDP 680 VQDEMLARFV 690 VGSHVRHHPS 700 NKEDGGLGGT 710 PEPAMPNTYG 720 VEPLPQEVLR 730 KYIIYAKEKV 740 HPKLNQMDQD 750 KVAKMYSDLR 760 KESMATGSIP 770 ITVRHIESMI 780 RMAEAHARIH 790 LRDYVMEDDV 800 NMAIRVMLES 810 FVDTQKFSVM 820 RSMRKTFARY 830 LSFRRDNNEL 840 LLFILKQLVA 850 EQVAYQRSRF 860 GAQQDTIEVP 870 EKDLVDKARQ 880 INIHNLSAFY 890 DSELFRMNKF 900 SHDLKRKVIL QQF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0042555 MCM complex
Cellular Component GO:0000228 nuclear chromosome
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0003678 DNA helicase activity
Molecular Function GO:0042393 histone binding
Molecular Function GO:0016787 hydrolase activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006270 DNA replication initiation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.