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Overview

Uniprot IDF1SPM8
Protein NameAP2-associated protein kinase 1
Gene NameAAK1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
169 VARLHQCKTPIIHRD
371 IAPRQRPKAGQTQPN

Function

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis (PubMed:11877457). Preferentially, may phosphorylate substrates on threonine residues (By similarity). Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes (By similarity). Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes (By similarity). Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (By similarity)

Protein Sequence

10 MKKFFDSRRE 20 QGGSGLGSGS 30 SGGGGSTSGL 40 GSGYIGRVFG 50 IGRQQVTVDE 60 VLAEGGFAIV 70 FLVRTSNGMK 80 CALKRMFVNN 90 EHDLQVCKRE 100 IQIMRDLSGH 110 KNIVGYIDSS 120 INNVSSGDVW 130 EVLILMDFCR 140 GGQVVNLMNQ 150 RLQTGFTESE 160 VLQIFCDTCE 170 AVARLHQCKT 180 PIIHRDLKVE 190 NILLHDRGHY 200 VLCDFGSATN 210 KFQNPQTEGV 220 NAVEDEIKKY 230 TTLSYRAPEM 240 VNLYSGKIIT 250 TKADIWALGC 260 LLYKLCYFTL 270 PFGESQVAIC 280 DGNFTIPDNS 290 RYSQDMHCLI 300 RYMLEPDPDK 310 RPDIYQVSFF 320 SFKLLKKECP 330 VPNVQNSPIP 340 AKLPEPVKAS 350 EAAAKKTQPK 360 ARLTDPIPTT 370 ETSIAPRQRP 380 KAGQTQPNPG 390 ILPIQPALTP 400 RKRAMVQPPP 410 QVAGSSNQPG 420 LLASVPQPKT 430 QAPPSQPLPQ 440 SQAKQPQAPP 450 APQQPPSAPA 460 QGLPAQAQAT 470 PQHQQQLFLK 480 QQPQPPQPQP 490 QAQAPPVKSL 500 KFYPFYPMCK 510 GRQTVSSQFQ 520 AVHPAAQQPA 530 IAQFPAVSQG 540 SSQQQLIQNF 550 YQQQQQQQQQ 560 QQLATTLHQQ 570 QLLTQQAALQ 580 QKTTVAAIQP 590 PQAQPATASQ 600 PPPAQEPAQI 610 QAPVRQQPKV 620 QTTPPPAIQG 630 QKLGSLTPPS 640 SPKAQRAGHR 650 RILSDVTHSA 660 VFGVPASKST 670 QLLQAAAAEA 680 SLNKSKSATT 690 TPSGSPRASQ 700 QNVYNPSEGS 710 TWNPFDDDNF 720 SKLTAEELLN 730 KDFAKLGEGK 740 HPEKLGGSAE 750 SLIPGFQPTQ 760 GDAFAASSFA 770 AGTAEKRKGG 780 QAVDSSLPLL 790 SVSDPFIPLQ 800 VPDAPEKLIE 810 GLKSPDTSLL 820 LPDLLPMTDP 830 FGSTSDAVIE 840 KADVAVESLI 850 PGLEPPVPQR 860 LPSQTESVAS 870 NRTDSLTGED 880 ALIDCSLLSN 890 PTTDLLEEFA 900 PIAISAPAHK 910 AAEDSNLISG 920 FDVPEGSDKV 930 AEDEFDPIPV 940 LITKNPQGGH 950 SRNSSGSSES 960 SLPNLARSLL LVDQLIDL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0031252 cell leading edge
Cellular Component GO:0005905 clathrin-coated pit
Cellular Component GO:0030136 clathrin-coated vesicle
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0098793 presynapse
Cellular Component GO:0043195 terminal bouton
Molecular Function GO:0035612 AP-2 adaptor complex binding
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0005112 Notch binding
Molecular Function GO:0106310 protein serine kinase activity
Molecular Function GO:0004674 protein serine/threonine kinase activity
Biological Process GO:0006897 endocytosis
Biological Process GO:0045747 positive regulation of Notch signaling pathway
Biological Process GO:0006468 protein phosphorylation
Biological Process GO:0050821 protein stabilization
Biological Process GO:2000369 regulation of clathrin-dependent endocytosis
Biological Process GO:0032880 regulation of protein localization

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.