Overview
| Uniprot ID | F1SQZ8 |
|---|
| Protein Name | tRNA ligase complex-associated NAD(P)H dehydrogenase PYROXD1 |
|---|
| Gene Name | PYROXD1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 109 |
GNQHVYKKLCLCAGA |
Function
Flavoprotein whose role is to protect RTCB, the catalytic subunit of the tRNA ligase complex (tRNA-LC) involved in tRNA splicing and unfolded protein response, from oxidative inactivation. It directly binds and shields the catalytic center of RTCB, prior to guanylylation, when it is inactive and its active site is vulnerable to oxidation under aerobic conditions. The interaction of PYROXD1 with RTCB is allosterically regulated by NAD(P)H binding and reduction of its FAD cofactor, while reoxidation of PYROXD1 thanks to its NADPH dehydrogenase activity triggers the timed release of RTCB, enabling its subsequent activation. Thereby, this protective mechanism may also function as a molecular timer, regulating RTCB activation through controlled release
Protein Sequence
10
MEVARSPPTA
20
GKFVVVGGGI
30
AGVTCAEQLA
40
IDFPSEDILL
50
ITASPVIKAV
60
TNFKQVSKVL
70
EEFDVEEQPS
80
TMLENRFPNI
90
KVIESGVKQL
100
KSEQRCILTE
110
DGNQHVYKKL
120
CLCAGAKPKL
130
ICEGNPYVLG
140
IRDTDSAQEF
150
QKQLTQAKRI
160
MIIGNGGIAL
170
ELVYEIEGCE
180
VIWVIKDKAI
190
GNTFFDAGAA
200
EFLTSKLIDE
210
KSEAKIAQKR
220
TRYTTEGRKK
230
EAQTKAHAGN
240
IGSALGPDWH
250
EGLDLKGTKE
260
FSHKIHIETM
270
CEVKNIYLQE
280
EFRISEKKSL
290
IFPKDHHNQS
300
VTTDKEIWPV
310
YVELTNEKIY
320
GCDFIVSATG
330
VTPNTEPFLC
340
GNNFDVGDDG
350
GLKVDDHMHT
360
SLPDIYAAGD
370
ICTASWQPSP
380
VWQQMRLWTQ
390
ARQMGWYAAK
400
CMAAASVGES
410
IDMDFSFELF
420
AHVTKFFNYK
430
VVLLGKYNAQ
440
GLGPDHELLL
450
RCTKGQEYIK
460
AVLQNGRMMG
470
AVLIGETDLE
480
ETFENLILNQ
490
MNLSAYGRFP
G
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0030017 |
sarcomere |
| Molecular Function |
GO:0140299 |
molecular sensor activity |
| Molecular Function |
GO:0016174 |
NAD(P)H oxidase H2O2-forming activity |
| Biological Process |
GO:0034599 |
cellular response to oxidative stress |
| Biological Process |
GO:0000968 |
tRNA exon ligation |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.