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Overview

Uniprot IDF1SUD5
Protein NameLysine-specific demethylase PHF2
Gene NamePHF2
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
566 PKKGKATKNVLSVPN

Function

Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. Involved in the activation of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages by catalyzing the demethylation of trimethylated histone H4 lysine 20 (H4K20me3) at the gene promoters

Protein Sequence

10 MATVPVYCVC 20 RLPYDVTRFM 30 IECDACKDWF 40 HGSCVGVEEE 50 EAPDIDIYHC 60 PNCEKTHGKS 70 TLKKKRTWHK 80 HSPGQTPDVK 90 PVQNGSQLFI 100 KELRSRTFPS 110 AEDVVARVPG 120 SQLTLGYMEE 130 HGFTEPILVP 140 KKDGLGLAVP 150 APTFYVSDVE 160 NYVGPERSVD 170 VTDVTKQKDC 180 KMKLKEFVDY 190 YYSTNRKRVL 200 NLTNLEFSDT 210 RMSSFVEPPD 220 IVKKLSWVEN 230 YWPDDALLAK 240 PKVTKYCLIC 250 VKDSYTDFHI 260 DSGGASAWYH 270 VLKGEKIFYL 280 IKPASANISL 290 YERWQSASNH 300 SEMFFADQVD 310 KCYKCTLKQG 320 QTLFIPSGWI 330 YATLTPVDCL 340 AFAGHFLHSL 350 SVEMQMRAYE 360 VERRLKLGSL 370 TQFPNFETAC 380 WYMGKHLLEA 390 FKGSHKSGKQ 400 LPPHLVQGAK 410 ILNGAFRSWT 420 KKQALAEHED 430 ELPEHFKPSQ 440 LIKDLAKEIR 450 LSENASKASR 460 PEVTAAVSSD 470 EVCFGDREKE 480 EPPSPIEASP 490 PRSFLEKVSK 500 KKTPKTVKMP 510 KPSKVPKPPK 520 PPKPPKSLKL 530 KDGGKKKGKK 540 CRGPASPTIP 550 NLDLLEAHTK 560 EALTKIEPPK 570 KGKATKNVLS 580 VPNKEVISTQ 590 NDVERLEIRE 600 QTKSKSEAKW 610 KYKNSKPDSL 620 LKMEEEQKLE 630 KSPLAGNKDT 640 KFSFSFSNKK 650 LLGSKTLKPQ 660 PSPGVFGALQ 670 NFKEDKPQPV 680 RDEYEYVSDD 690 GELKIDEFPI 700 RRKKNAPKRD 710 LSFLLDKKEP 720 LPTPTSKPKL 730 DSALYKQSDD 740 SSDEGSLHID 750 TDTKPARNAK 760 VKKDGGGSAA 770 GILDLLQASE 780 EVGALEYNPN 790 SQPPASPSTQ 800 EAIQGMLSMA 810 NLQASDSCLQ 820 TAWGAGQAKG 830 SSLAAHGPRK 840 NGAGGKSAGK 850 RLLKRTAKNS 860 VDLDDYEEEQ 870 DHLDACFKDS 880 DYVYPSLESD 890 EDSPVFKSRS 900 KKRKGSDDAP 910 YSPTARVGPS 920 VPRQDRPVRE 930 GTRVASIETG 940 LAAAAAKLSQ 950 QEEQKNRKKK 960 NTKRKLAPNT 970 TSPSASVTTL 980 AGTTSASTTS 990 ASTTSASTTS 1000 ASTTSASTTP 1010 ASTTPASTST 1020 ASSQASQEGS 1030 SPEPLPESHS 1040 SSLVDHEYTA 1050 AGAFAGAQAG 1060 RSSQPMAPGV 1070 FLTQRRPSSS 1080 SPNNTAAKGK 1090 RTKKGMATAK 1100 QRLGKILKIH RNGKLLL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0005730 nucleolus
Molecular Function GO:0032452 histone demethylase activity
Molecular Function GO:0140002 histone H3K4me3 reader activity
Molecular Function GO:0032454 histone H3K9 demethylase activity
Molecular Function GO:0035575 histone H4K20 demethylase activity
Molecular Function GO:0005506 iron ion binding
Molecular Function GO:0003713 transcription coactivator activity
Molecular Function GO:0003712 transcription coregulator activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0061188 negative regulation of rDNA heterochromatin formation
Biological Process GO:0006357 regulation of transcription by RNA polymerase II
Biological Process GO:0045815 transcription initiation-coupled chromatin remodeling

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.