Overview
| Uniprot ID | F2Z5R2 |
|---|
| Protein Name | GTP-binding protein Rheb |
|---|
| Gene Name | RHEB |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 8 |
MPQSKSRKIAILGYR |
| 97 |
IKSFEVIKVIHGKLL |
Function
Small GTPase that acts as an allosteric activator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In response to nutrients, growth factors or amino acids, specifically activates the protein kinase activity of MTOR, the catalytic component of the mTORC1 complex: acts by causing a conformational change that allows the alignment of residues in the active site of MTOR, thereby enhancing the phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). RHEB is also required for localization of the TSC-TBC complex to lysosomal membranes. In response to starvation, RHEB is inactivated by the TSC-TBC complex, preventing activation of mTORC1. Has low intrinsic GTPase activity
Protein Sequence
10
MPQSKSRKIA
20
ILGYRSVGKS
30
SLTIQFVEGQ
40
FVDSYDPTIE
50
NTFTKLITVN
60
GQEYHLQLVD
70
TAGQDEYSIF
80
PQTYSIDING
90
YILVYSVTSI
100
KSFEVIKVIH
110
GKLLDMVGKV
120
QIPIMLVGNK
130
KDLHMERVIS
140
YEEGKALAES
150
WNAAFLESSA
160
KENQTAVDVF
170
RRIILEAEKI
180
DGAASQGKSS
CSVM
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0000139 |
Golgi membrane |
| Cellular Component |
GO:0005765 |
lysosomal membrane |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0005525 |
GTP binding |
| Molecular Function |
GO:0003924 |
GTPase activity |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0043539 |
protein serine/threonine kinase activator activity |
| Biological Process |
GO:1904263 |
positive regulation of TORC1 signaling |
| Biological Process |
GO:0007264 |
small GTPase-mediated signal transduction |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.