Search Results

Overview

Uniprot ID	F6Q6B4
Protein Name	Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Gene Name	PCK2
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
141	DVARVESKTVIVTPS
294	GGNSLLGKKCFALRI

Function

Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Can play an active role in glyceroneogenesis and gluconeogenesis. Also acts as a serine/threonine-protein kinase: phosphorylates and activates ACSL4, thereby promoting ferroptosis

Protein Sequence

10 MAAMYRPGLR 20 CNSCAPGWAR 30 STRALGTRVH 40 EPLGIELPEK 50 GRRLSWHGLS 60 SWGWSSRRSI 70 QALRVLNGDL 80 GQLPAGVRDF 90 VERSARLCQP 100 EGIHICDGTE 110 AENTATLALL 120 EQQGLIRKLP 130 KYNNCWLART 140 DPKDVARVES 150 KTVIVTPSQR 160 DTVPLPAGGA 170 RGQLGNWMSP 180 AEFQQAVDER 190 FPGCMQGRTM 200 YVLPFSMGPV 210 GSPLSRIGVQ 220 LTDSAYVVAS 230 MRIMTRLGKP 240 VLQALGDGDF 250 VKCLHSVGQP 260 LTGQGEPVSQ 270 WPCNPEKTLI 280 GHVPDQREIV 290 SFGSGYGGNS 300 LLGKKCFALR 310 IASRLARDEG 320 WLAEHMLILG 330 ITNPAGKKRY 340 VAAAFPSACG 350 KTNLAMMRPA 360 LPGWKVECVG 370 DDIAWMRFDS 380 DGQLRAINPE 390 NGFFGVAPGT 400 SATTNPNAMA 410 TIQSNTLFTN 420 VAETSDGGVY 430 WEGIDQPLPP 440 GVTVTSWLGK 450 PWKPGDKEPC 460 AHPNSRFCAP 470 ARQCPIMDPA 480 WEAPEGVPID 490 AIIFGGRRPK 500 GKVIMHDPFA 510 MRPFFGYNFG 520 RYLEHWLSME 530 GRKGARLPRI 540 FHVNWFRRDE 550 AGHFLWPGFG 560 ENARVLDWIC 570 RRLEQEDSAR 580 ETPIGLVPKE 590 GALDLRGLGA 600 IDTTQLFSIP 610 KDFWEQEVRD 620 IRSYLTEQVN 630 QDLPKEVLAE 640 LEALEGRVRR M

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:0005525	GTP binding
Molecular Function	GO:0016301	kinase activity
Molecular Function	GO:0046872	metal ion binding
Molecular Function	GO:0004613	phosphoenolpyruvate carboxykinase (GTP) activity
Biological Process	GO:0006094	gluconeogenesis

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.