Search Results
Overview
| Uniprot ID | F7EMB2 |
|---|---|
| Protein Name | tRNA-splicing ligase complex subunit RTRAF |
| Gene Name | Rtraf |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 171 | LTQDAVAKANQMKEG |
| 176 | VAKANQMKEGLPVAL |
| 98 | EYGDNAEKYKDSVPD |
Function
Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Could also play a role in RNA transport
Protein Sequence
10
MFRRKLTALD
20
YHNPTGFNCK
30
DETEFRNFIV
40
WLEDQKIRHY
50
KIEDRGNLRN
60
IHSSDWPKFF
70
EKYLRDVNCP
80
FKIQDRQEAI
90
DWLLGLAVRL
100
EYGDNAEKYK
110
DSVPDNRKNA
120
DNAAKNSEPL
130
INLDVNNPDF
140
KAGVMALANL
150
LQIQRHDDYL
160
VMLKAIRILV
170
QERLTQDAVA
180
KANQMKEGLP
190
VALDKHILGF
200
DTGDAVLNEA
210
AQILRLLHIE
220
ELRELQTKIN
230
EAIVAVQAII
240
ADPKTDHRLG
KVGR
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0005813 | centrosome |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0072686 | mitotic spindle |
| Cellular Component | GO:0005654 | nucleoplasm |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0048471 | perinuclear region of cytoplasm |
| Cellular Component | GO:0072669 | tRNA-splicing ligase complex |
| Molecular Function | GO:0003723 | RNA binding |
| Molecular Function | GO:0000993 | RNA polymerase II complex binding |
| Biological Process | GO:0006469 | negative regulation of protein kinase activity |
| Biological Process | GO:0045944 | positive regulation of transcription by RNA polymerase II |
| Biological Process | GO:0006388 | tRNA splicing, via endonucleolytic cleavage and ligation |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.
[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.