Overview
| Uniprot ID | G3V8C4 |
|---|
| Protein Name | Chloride intracellular channel protein |
|---|
| Gene Name | Clic4 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 146 |
ALERGLLKTLQKLDE |
| 199 |
LPKLHIVKVVAKKYR |
| 249 |
IAYSDVAKRLTK*** |
Function
In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Promotes cell-surface expression of HRH3
Protein Sequence
10
MALSMPLNGL
20
KEEDKEPLIE
30
LFVKAGSDGE
40
SIGNCPFSQR
50
LFMILWLKGV
60
VFSVTTVDLK
70
RKPADLQNLA
80
PGTHPPFITF
90
NSEVKTDVNK
100
IEEFLEEVLC
110
PPKYLKLSPK
120
HPESNTAGMD
130
IFAKFSAYIK
140
NSRPEANEAL
150
ERGLLKTLQK
160
LDEYLNSPLP
170
DEIDENSMED
180
IKSSTRRFLD
190
GDEMTLADCN
200
LLPKLHIVKV
210
VAKKYRNFDI
220
PKGMTGIWRY
230
LTNAYSRDEF
240
TNTCPSDKEV
250
EIAYSDVAKR
LTK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0045177 |
apical part of cell |
| Cellular Component |
GO:0009986 |
cell surface |
| Cellular Component |
GO:0005911 |
cell-cell junction |
| Cellular Component |
GO:0005813 |
centrosome |
| Cellular Component |
GO:0034707 |
chloride channel complex |
| Cellular Component |
GO:0030659 |
cytoplasmic vesicle membrane |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005902 |
microvillus |
| Cellular Component |
GO:0030496 |
midbody |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0016363 |
nuclear matrix |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0005254 |
chloride channel activity |
| Molecular Function |
GO:0016491 |
oxidoreductase activity |
| Biological Process |
GO:0071277 |
cellular response to calcium ion |
| Biological Process |
GO:0030216 |
keratinocyte differentiation |
| Biological Process |
GO:0030336 |
negative regulation of cell migration |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.