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Overview

Uniprot IDH0UVA7
Protein NameNucleolin
Gene NameNCL
OrganismCavia porcellus

Kla Sites from experimental identification

Position Flanking peptide
101 KKTVTPAKAVVAPGK
115 KKGAALGKAVVATPG
123 AVVATPGKKGTVTPA
229 KGKKAPAKAAPVKAK
401 DARTLLAKNLPYKVT
406 LAKNLPYKVTQDELK
432 VSKDGKSKGIAYIEF
470 SLYYTGEKGQNQDYR
480 NQDYRGGKNSTWSGE
548 EALNSCNKREIEGRT
575 NARSQPSKTLFVKGL
580 PSKTLFVKGLSEETT
61 AAVTPAKKAVASPVK
613 DRETGSSKGFGFVDF
68 KAVASPVKKVAVATP
69 AVASPVKKVAVATPA
77 VAVATPAKKAAVTPG

Function

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats

Protein Sequence

10 MVKLAKAGKN 20 QSDPKKMAPP 30 PKEVEESDDE 40 EMSDDEDDSS 50 GEEVVIPQKK 60 SKKAAVTPAK 70 KAVASPVKKV 80 AVATPAKKAA 90 VTPGKKAAAA 100 TPAKKTVTPA 110 KAVVAPGKKG 120 AALGKAVVAT 130 PGKKGTVTPA 140 KGAKNGKNAK 150 KEDSDEDEED 160 GDSDEEDDDE 170 EEEEEEDEEF 180 EPKVMKAVAA 190 APASEDEDED 200 DDDDDDDDEE 210 EDDDDDEDDD 220 SEEEAMETTP 230 AKGKKAPAKA 240 APVKAKSMAE 250 EEDDDEDDDD 260 EDEDEDDEED 270 EDEEEEEEEE 280 EEEEPVKEAP 290 GKRKKDMTKQ 300 KAAPEAKKQK 310 MEGTEPTTAF 320 NLFVGNLNSS 330 KSPSELKTGI 340 SEVFAKNDLT 350 TVDVRIGTNR 360 KFGYVDFESA 370 EDLEKALELT 380 GLKVFGSEIK 390 LEKPKGKDSK 400 KDRDARTLLA 410 KNLPYKVTQD 420 ELKEVFEDAL 430 EIRLVSKDGK 440 SKGIAYIEFK 450 TEADAEKTFE 460 EKQGTEIDGR 470 SVSLYYTGEK 480 GQNQDYRGGK 490 NSTWSGESKT 500 LYLSNLSYSA 510 TEETLQEVFE 520 KATFIKVPQN 530 QNGKSKGYAF 540 IEFASFEDAK 550 EALNSCNKRE 560 IEGRTIRLEL 570 QGPRGSPNAR 580 SQPSKTLFVK 590 GLSEETTEET 600 LKESFDGSIG 610 ARIVTDRETG 620 SSKGFGFVDF 630 NTEEDAKAAK 640 EAMEDGEIDG 650 NKVTLDWAKP 660 KGEGGFGGRG 670 GGRGGFGGRG 680 GGRGGRGGFG 690 GRGRGGFGGR 700 GGFRGGRGGG 710 GDHKPQGKKT KFE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005938 cell cortex
Cellular Component GO:0005694 chromosome
Cellular Component GO:0001533 cornified envelope
Cellular Component GO:0036464 cytoplasmic ribonucleoprotein granule
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:1990904 ribonucleoprotein complex
Molecular Function GO:0044547 DNA topoisomerase binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0043560 insulin receptor substrate binding
Molecular Function GO:0048027 mRNA 5'-UTR binding
Molecular Function GO:0042731 PH domain binding
Molecular Function GO:0042162 telomeric DNA binding
Biological Process GO:0001525 angiogenesis
Biological Process GO:0017148 negative regulation of translation
Biological Process GO:1901838 positive regulation of transcription of nucleolar large rRNA by RNA polymerase I

Reference

[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.