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Overview

Uniprot IDH0UXE0
Protein NamePre-mRNA-splicing factor RBM22
Gene NameRBM22
OrganismCavia porcellus

Kla Sites from experimental identification

Position Flanking peptide
149 STSDMLLKLARTTPY
212 INDPVADKLLKRAST

Function

Required for pre-mRNA splicing as component of the activated spliceosome. Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses

Protein Sequence

10 MATSLGSNTY 20 NRQNWEDADF 30 PILCQTCLGE 40 NPYIRMTKEK 50 YGKECKICAR 60 PFTVFRWCPG 70 VRMRFKKTEV 80 CQTCSKLKNV 90 CQTCLLDLEY 100 GLPIQVRDAG 110 LSFKDDMPKS 120 DVNKEYYTQN 130 MEREISNSDG 140 TRPVGMLGKA 150 TSTSDMLLKL 160 ARTTPYYKRN 170 RPHICSFWVK 180 GECKRGEECP 190 YRHEKPTDPD 200 DPLADQNIKD 210 RYYGINDPVA 220 DKLLKRASTM 230 PRLDPPEDKT 240 ITTLYVGGLG 250 DTITETDLRN 260 HFYQFGEIRT 270 ITVVQRQQCA 280 FIQFATRQAA 290 EVAAEKSFNK 300 LIVNGRRLNV 310 KWGRSQAARG 320 KEKEKDGTTD 330 SGIKLEPVPG 340 LPGALPPPPA 350 AEEEASANYF 360 NLPPSGPPAV 370 VNIALPPPPG 380 IAPPPPPGFG 390 PHMFHPMGPP 400 PPFMRAPGPI 410 HYPSQDPQRM 420 GAHAGKHSSP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0000974 Prp19 complex
Cellular Component GO:0071006 U2-type catalytic step 1 spliceosome
Cellular Component GO:0071007 U2-type catalytic step 2 spliceosome
Molecular Function GO:0048306 calcium-dependent protein binding
Molecular Function GO:0036002 pre-mRNA binding
Molecular Function GO:0017070 U6 snRNA binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0071466 cellular response to xenobiotic stimulus
Biological Process GO:0045292 mRNA cis splicing, via spliceosome
Biological Process GO:0046827 positive regulation of protein export from nucleus
Biological Process GO:0042307 positive regulation of protein import into nucleus
Biological Process GO:0033120 positive regulation of RNA splicing

Reference

[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.