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Overview

Uniprot IDH0UYU8
Protein NameMitotic spindle assembly checkpoint protein MAD1
Gene NameMAD1L1
OrganismCavia porcellus

Kla Sites from experimental identification

Position Flanking peptide
61 RAEQIRSKSYLIQVE
87 RARVELEKAASTSAR

Function

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling

Protein Sequence

10 MEDLGENTTV 20 LSTLRSLNNF 30 ISQRVEGESG 40 LDTSTSASGS 50 LQMQYHQSIQ 60 LEERAEQIRS 70 KSYLIQVERE 80 KMQMELSHKR 90 ARVELEKAAS 100 TSARNYEREV 110 DRNQELLARL 120 RQYQDREAEA 130 EEKMREQLER 140 HRVCRQNLDS 150 ASQQLREQEN 160 SLAVASETIG 170 TLKGQVSELQ 180 WSLMDQEVQV 190 KRLESEKQEL 200 QEQLDLQHRK 210 WQEANQKIQE 220 LQASQEAKAD 230 QEQKIKDLEQ 240 KLCLQEQDAA 250 VVRNMKSELL 260 RLPRVERELK 270 RLREENAHLR 280 EMRDTNGLLM 290 EELEGLQRRL 300 GRQEKMQEAL 310 VGLELEKERL 320 LTKLQSWEIL 330 DQTTGLNLRT 340 PEDLSNFIVE 350 LQQRELALKE 360 KNSAITISAR 370 GLEKAQQQLQ 380 EEVRQVSGQL 390 LEERKKRENH 400 EALARRLQKR 410 VLLLTKERDG 420 MRAILGSYDS 430 ELTQAEYSPQ 440 LTRRMREAED 450 MVQKMHAHSS 460 EMEAQLSQAM 470 EELGGQKQRA 480 DMLEMELKML 490 KSQPSSAEPS 500 FPFCREEMDT 510 LRSKVEELEA 520 ERSRLEQEKN 530 VLEMQLERRT 540 LQGDYDQSKT 550 KVLHMSLNPT 560 SLAKQRLREE 570 RDRLQEECER 580 LRGLVHALER 590 GGPVPADLEA 600 TTGLASSKEV 610 AELRKQVESA 620 ELKNQRLKEV 630 FQTKIQEFRK 640 VCYTLTGYQI 650 DITTENQYRL 660 TSQYAEHKTD 670 CLIFKATGPS 680 GSKMQLLETE 690 FSRSVPELIE 700 LHLLRQDSIP 710 AFLSALTLEL FSHQTAA

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005813 centrosome
Cellular Component GO:0000776 kinetochore
Cellular Component GO:1990706 MAD1 complex
Cellular Component GO:1990728 mitotic spindle assembly checkpoint MAD1-MAD2 complex
Cellular Component GO:0097431 mitotic spindle pole
Cellular Component GO:0031965 nuclear membrane
Cellular Component GO:0044615 nuclear pore nuclear basket
Cellular Component GO:0005654 nucleoplasm
Molecular Function GO:0043515 kinetochore binding
Biological Process GO:0051315 attachment of mitotic spindle microtubules to kinetochore
Biological Process GO:0051301 cell division
Biological Process GO:0007094 mitotic spindle assembly checkpoint signaling
Biological Process GO:0090267 positive regulation of mitotic cell cycle spindle assembly checkpoint
Biological Process GO:0090235 regulation of metaphase plate congression

Reference

[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.