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Overview

Uniprot IDH0VDS9
Protein NameDNA topoisomerase I
Gene NameTOP1
OrganismCavia porcellus

Kla Sites from experimental identification

Position Flanking peptide
140 KEDLKPLKRPRDEDD
153 DDADYKPKKIKTEDI
156 DYKPKKIKTEDIKKE
167 IKKEKKRKLEEEEDG
175 LEEEEDGKLKKPKNK

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone

Protein Sequence

10 MSGDHLHNDS 20 QIEADFRLND 30 SHKHKDKHKD 40 REHRHKEHKK 50 DKEKDREKSK 60 HSNSEHKDSE 70 KKHKEKEKTK 80 HKDGSSEKHK 90 DKHKDRDKEK 100 RKEEKIRASG 110 DAKIKKEKEN 120 GFSSPPRIKD 130 EPEDDGYFVP 140 PPKEDLKPLK 150 RPRDEDDADY 160 KPKKIKTEDI 170 KKEKKRKLEE 180 EEDGKLKKPK 190 NKDKEKKLPE 200 PDNKKKKPKK 210 EEEQKWKWWE 220 EERYPEGIKW 230 KFLEHKGPVF 240 APPYEPLPES 250 VKFYYDGKVM 260 KLSPKAEEIA 270 TFFAKMLDHE 280 YTTKEIFRKN 290 FFKDWRKEMT 300 NEEKNTITNL 310 SKCDFTQMSQ 320 YFKAQSEARK 330 QMSKEEKLKI 340 KEENEKLLKE 350 YGFCVMDNHR 360 ERIANFKIEP 370 PGLFRGRGNH 380 PKMGMLKRRI 390 LPEDIIINCS 400 KDAKVPSPPP 410 GHKWKEVRHD 420 NKVTWLVSWT 430 ENIQGSIKYI 440 MLNPSSRIKG 450 EKDWQKYETA 460 RRLKKCVDKI 470 RNQYREDWKS 480 KEMKVRQRAV 490 ALYFIDKLAL 500 RAGNEKEEGE 510 TADTVGCCSL 520 RVEHINLHPE 530 LDGQEYVVEF 540 DFLGKDSIRY 550 YNKVPVEKRV 560 FKNLQLFMEN 570 KQPEDDLFDR 580 LNTGILNKHL 590 QDLMEGLTAK 600 VFRTYNASIT 610 LQQQLKELTA 620 PDENIPAKIL 630 SYNRANRAVA 640 ILCNHQRAPP 650 KTFEKSMMNL 660 QSKIDAKKDQ 670 LADARRDLKS 680 AKADAKVMKD 690 AKTKKVVESK 700 KKAVQRLEEQ 710 LMKLEVQATD 720 REENKQVALG 730 TSKLNYLDPR 740 ITVAWCKKWG 750 VPIEKIFNKT 760 QREKFAWAID MADEDYEF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0001650 fibrillar center
Cellular Component GO:0000228 nuclear chromosome
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0000932 P-body
Cellular Component GO:0043204 perikaryon
Cellular Component GO:0032993 protein-DNA complex
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Function GO:0140693 molecular condensate scaffold activity
Molecular Function GO:0019904 protein domain specific binding
Molecular Function GO:0004674 protein serine/threonine kinase activity
Molecular Function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function GO:0003697 single-stranded DNA binding
Molecular Function GO:0097100 supercoiled DNA binding
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0007059 chromosome segregation
Biological Process GO:0032922 circadian regulation of gene expression
Biological Process GO:0006260 DNA replication
Biological Process GO:0006265 DNA topological change
Biological Process GO:0009410 response to xenobiotic stimulus

Reference

[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.