Overview
| Uniprot ID | H0VRD6 |
| Protein Name | Splicing factor 3A subunit 1 |
| Gene Name | SF3A1 |
| Organism | Cavia porcellus |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 424 |
GEKIPASKMQEHMRI |
| 533 |
IEAIHKAKGLVPEDD |
| 702 |
EEFLRRNKGPVSIKV |
Function
Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A1 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes
Protein Sequence
10
MPAGPVQAVP
20
PPPPVITEPK
30
QPTEEEGSSK
40
EDSTPSKPVV
50
GIIYPPPEVR
60
NIVDKTASFV
70
ARNGPEFEAR
80
IRQNEINNPK
90
FNFLNPNDPY
100
HAYYRHKVSE
110
FKEGKAQEPS
120
AAIPKVMQQQ
130
QQAAQQQLPQ
140
KVQAQVIQET
150
IVPKEPPPEF
160
EFIADPPSIS
170
AFDLDVVKLT
180
AQFVARNGRQ
190
FLTQLMQKEQ
200
RNYQFDFLRP
210
QHSLFNYFTK
220
LVEQYTKILI
230
PPKGLFTKLK
240
KEAENPREVL
250
DQVCYRVEWA
260
KFQERERKKE
270
EEEKEKERVA
280
YAQIDWHDFV
290
VVETVDFQPN
300
EQGNFPPPTT
310
PEELGARILI
320
QERYEKFGES
330
EEVEMEVESD
340
EEDEKQEKSE
350
ETPSQLDQDT
360
QVQDMDEGSD
370
DEEEGQKVPP
380
PPETPMPPPL
390
PPTPDQVIVR
400
KDYDPKASKP
410
LPPAPAPDEY
420
LVSPITGEKI
430
PASKMQEHMR
440
IGLLDPRWLE
450
QRDRSIREKQ
460
SDDEVYAPGL
470
DIESSLKQLA
480
ERRTDIFGVE
490
ETAIGKKIGE
500
EEIQKPEEKV
510
TWDGHSGSMA
520
RTQQAAQANI
530
TLQEQIEAIH
540
KAKGLVPEDD
550
TKEKIGPSKP
560
NEIPQQPPPP
570
SSATNIPSSA
580
PPITSVPRPP
590
AMPPPVRTTV
600
VSAVPVMPRP
610
PMASVVRLPP
620
GSVIAPMPPI
630
IHAPRINVVP
640
MPPSAPPIMA
650
PRPPPMIVPT
660
AFVPAPPVAP
670
VPAPAPMPPV
680
HPPPPMEDEP
690
ASKKLKTEDS
700
LMPEEEFLRR
710
NKGPVSIKVQ
720
VPNMQDKTEW
730
KLNGQVLVFT
740
LPLTDQVSVI
750
KVKIHEATGM
760
PAGKQKLQYE
770
GIFIKDSNSL
780
AYYNMANGAV
790
IHLALKERGG
RKK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0071013 |
catalytic step 2 spliceosome |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Cellular Component |
GO:0005686 |
U2 snRNP |
| Cellular Component |
GO:0071005 |
U2-type precatalytic spliceosome |
| Cellular Component |
GO:0071004 |
U2-type prespliceosome |
| Molecular Function |
GO:0003723 |
RNA binding |
| Biological Process |
GO:0045292 |
mRNA cis splicing, via spliceosome |
| Biological Process |
GO:0000381 |
regulation of alternative mRNA splicing, via spliceosome |
| Biological Process |
GO:1903241 |
U2-type prespliceosome assembly |
Reference
[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.