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Overview

Uniprot IDH0VS89
Protein NameSuccinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
Gene NameSUCLA2
OrganismCavia porcellus

Kla Sites from experimental identification

Position Flanking peptide
102 VLAGGRGKGTFESGL

Function

ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. Also able to act as an ATP-specific itaconyl- and malyl-CoA synthetase

Protein Sequence

10 MAALLASPSL 20 RSHRPRTALR 30 AAAQVLGSSG 40 LFNNHGLQIQ 50 QQQQRNLSLH 60 EYMSMELLQE 70 AGVSIPKGYV 80 AKSADEAYAI 90 AKKLGSKDVV 100 IKAQVLAGGR 110 GKGTFESGLK 120 GGVKIVFSPE 130 EAKAVSSQMI 140 GKKLFTKQTG 150 AKGRICNQVL 160 ICERKYPRRE 170 YYFAITMERS 180 FQGPVLIGSS 190 QGGVNIEDVA 200 AETPDAIVKE 210 PVDIVEGIKK 220 EQAVQLATKM 230 GFPANIVDSA 240 AENMVKLYNL 250 FLKYDATMVE 260 INPMVEDSDG 270 AVLCMDAKIN 280 FDSNSAYRQK 290 KIFDLQDWTQ 300 EDERDRDAAK 310 ANLNYIGLDG 320 NIGCLVNGAG 330 LAMATMDIIK 340 LHGGTPANFL 350 DVGGGATVQQ 360 VTEAFKLITS 370 DKKVLAILVN 380 IFGGIMRCDV 390 IAQGIVMAVK 400 DLEIKIPVVV 410 RLQGTRVDDA 420 KALIADSGLK 430 ILACDDLDEA 440 AKMVVKLSEI 450 VTLAKQAHVD VKFQLPI

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0009361 succinate-CoA ligase complex (ADP-forming)
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0004775 succinate-CoA ligase (ADP-forming) activity
Biological Process GO:0006108 malate metabolic process
Biological Process GO:0006104 succinyl-CoA metabolic process
Biological Process GO:0110052 toxic metabolite repair
Biological Process GO:0006099 tricarboxylic acid cycle

Reference

[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.