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Overview

Uniprot ID	H0VTB1
Protein Name	Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Gene Name	DHX16
Organism	Cavia porcellus

Kla Sites from experimental identification

Position	Flanking peptide
1029	ELEDPNAKKMPKKIG

Function

Required for pre-mRNA splicing as a component of the spliceosome. Contributes to pre-mRNA splicing after spliceosome formation and prior to the first transesterification reaction. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. Also plays a role in innate antiviral response by acting as a pattern recognition receptor sensing splicing signals in viral RNA. Mechanistically, TRIM6 promotes the interaction between unanchored 'Lys-48'-polyubiquitin chains and DHX16, leading to DHX16 interaction with RIGI and ssRNA to amplify RIGI-dependent innate antiviral immune responses

Protein Sequence

10 MATPAALERW 20 VLEELHTVLG 30 LSKRHVAQFL 40 IGTAQRCASA 50 EEFVQRLRDT 60 DTLDLDGPAR 70 DFALRLWSKV 80 PRKAAVEKPA 90 RAAEREARAL 100 LEKNRSYRLL 110 EDSEDSSGEI 120 AGRAGSSPQK 130 KRRKRKHLRK 140 KRQEEEEEEE 150 VASEGGSKAA 160 GSRQQTEKAP 170 ESEDEWERTE 180 RERLQDLEER 190 DAFAERVRQR 200 DKERTRNVLE 210 RSDKKAYEEA 220 QKRLKMAEED 230 QKAMVPELRK 240 KSRREYLAKR 250 EREKLEDLEA 260 ELADEEYLFG 270 DVELSRHERR 280 ELRYKRRVRD 290 LAREYRAAGE 300 QEKLEATNRY 310 HMPKETRGQP 320 SRAVDLVEEE 330 SGAPGEEQRR 340 WEEARLGAAA 350 LKFGARDAAA 360 QEPQYQLVLE 370 EEEAIEFVRA 380 TQLQGDEEPV 390 APAAAVQAQQ 400 KESIQAVRRS 410 LPVFPFREEL 420 LAAVAHHQVL 430 IIEGETGSGK 440 TTQIPQYLFE 450 EGYTAKGMKI 460 ACTQPRRVAA 470 MSVAARVARE 480 MGVKLGNEVG 490 YSIRFEDCTS 500 ERTVLRYMTD 510 GMLLREFLSE 520 PDLASYSVVM 530 VDEAHERTLH 540 TDILFGLIKD 550 VARFRPELKV 560 LVASATLDTA 570 RFSTFFDDAP 580 VFRIPGRRFP 590 VDIFYTKAPE 600 ADYLEACVVS 610 VLQIHVTQPP 620 GDILVFLTGQ 630 EEIEAACEML 640 QDRCRRLGSK 650 IRELLVLPIY 660 ANLPSDMQAR 670 IFQPTPPGAR 680 KVVVATNIAE 690 TSLTIEGIIY 700 VLDPGFCKQK 710 SYNPRTGMES 720 LTVTPCSKAS 730 ANQRAGRAGR 740 VAAGKCFRLY 750 TAWAYQHELE 760 ETTVPEIQRT 770 SLGNVVLLLK 780 SLGIHDLMHF 790 DFLDPPPYET 800 LLLALEQLYA 810 LGALNHLGEL 820 TTSGRKMAEL 830 PVDPMLSKMI 840 LASEKYSCSE 850 EILTVAAMLS 860 VNNSIFYRPK 870 DKVVHADNAR 880 VNFFLPGGDH 890 LVLLNVYTQW 900 AESGYSSQWC 910 YENFVQFRSM 920 RRARDVREQL 930 EGLLERVEVG 940 LSSCQGDYVR 950 VRKAITAGYF 960 YHTARLTRSG 970 YRTVKQQQTV 980 FIHPNSSLFE 990 QQPRWLLYHE 1000 LVLTTKEFMR 1010 QVLEIESSWL 1020 LEVAPHYYKA 1030 KELEDPNAKK 1040 MPKKIGKTRE ELG

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0071013	catalytic step 2 spliceosome
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005654	nucleoplasm
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016787	hydrolase activity
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0003724	RNA helicase activity
Biological Process	GO:0045087	innate immune response
Biological Process	GO:0000398	mRNA splicing, via spliceosome

Reference

[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.