Overview
| Uniprot ID | H0W2M4 |
|---|
| Protein Name | Ketimine reductase mu-crystallin |
|---|
| Gene Name | CRYM |
|---|
| Organism | Cavia porcellus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 176 |
RTKENAEKFANTVQG |
| 184 |
FANTVQGKVRVCSSV |
| 286 |
VKSAHCEKTTVFKSL |
| 54 |
RTVVPVSKHRGFLGV |
Function
Catalyzes the NAD(P)H-dependent reduction of imine double bonds of a number of cyclic ketimine substrates, including sulfur-containing cyclic ketimines. Under physiological conditions, it efficiently catalyzes delta(1)-piperideine-2-carboxylate (P2C) and delta(1)-pyrroline-2-carboxylate (Pyr2C) reduction, suggesting a central role in lysine and glutamate metabolism. Additional substrates are delta(2)-thiazoline-2-carboxylate (T2C), 3,4-dehydrothiomorpholine-3-carboxylate (AECK), and (R)-lanthionine ketimine (LK) that is reduced at very low rate compared to other substrates. Also catalyzes the NAD(P)H-dependent reduction of (S)-cystathionine ketimine (CysK)
Protein Sequence
10
MSRAPAFLSA
20
AEVQDHLRSS
30
SLLIPPLEAA
40
LANFSSGLDG
50
GVVQPVRTVV
60
PVSKHRGFLG
70
VMPVYSAAED
80
ALTTKLVTFY
90
EGHNTSSAVP
100
SHQAIVLLFE
110
PSNGSLLAVM
120
DGNVITAKRT
130
AAVSAIATKF
140
LKPPGSDVLC
150
ILGAGVQAYS
160
HYEIFTEQFP
170
FKEVRIWNRT
180
KENAEKFANT
190
VQGKVRVCSS
200
VQEAVTGADV
210
IITVTMATEP
220
ILFGEWVKPG
230
AHINAVGASR
240
PDWRELDDEL
250
MKQAVLYVDS
260
REAALKESGD
270
VLLSGAEIFA
280
ELGEVINGVK
290
SAHCEKTTVF
300
KSLGMAVEDM
310
VAAKLIYDSW
SSGK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0047125 |
delta1-piperideine-2-carboxylate reductase activity |
| Molecular Function |
GO:0050661 |
NADP binding |
| Molecular Function |
GO:0042803 |
protein homodimerization activity |
| Molecular Function |
GO:0050241 |
pyrroline-2-carboxylate reductase activity |
| Molecular Function |
GO:0047127 |
thiomorpholine-carboxylate dehydrogenase activity |
| Molecular Function |
GO:0070324 |
thyroid hormone binding |
| Molecular Function |
GO:0003714 |
transcription corepressor activity |
| Biological Process |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0007605 |
sensory perception of sound |
| Biological Process |
GO:0042403 |
thyroid hormone metabolic process |
| Biological Process |
GO:0070327 |
thyroid hormone transport |
Reference
[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.