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Overview

Uniprot IDH0WBZ0
Protein NameSUMO-activating enzyme subunit 1
Gene NameSAE1
OrganismCavia porcellus

Kla Sites from experimental identification

Position Flanking peptide
184 EEKTKVAKVSQGVED
196 VEDGPDTKRAKLDSS
199 GPDTKRAKLDSSETT

Function

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2

Protein Sequence

10 GRVAASARRE 20 AAQYDRQIRL 30 WGLEAQKTVR 40 AVVALRASRV 50 LLVGMKGLGA 60 EIAKNLILAG 70 VKGLTMLDHE 80 QVSPDDPGAQ 90 FLIRTGSIGR 100 NRAEASLERA 110 QNLNPMVDVK 120 VDTEDIEKKP 130 ESFFTQFDAV 140 CLTCCSRDVI 150 VKVDQICHKN 160 SIKFFTGDVF 170 GYHGYTFANL 180 GEHEFVEEKT 190 KVAKVSQGVE 200 DGPDTKRAKL 210 DSSETTMVKK 220 KVVFCPVKEA 230 LEVDWSSEKA 240 KAALKRTTSD 250 YFLLQVLLKF 260 RTDKGRDPGS 270 DTYGEDSELL 280 LHIRNDVLDS 290 LGVSPDLLPD 300 DFVRYCFSEM 310 APVCAVVGGI 320 LAQEIVKALS 330 QRDPPHNNFF 340 FFDGMKGNGI VECLGPK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0031510 SUMO activating enzyme complex
Molecular Function GO:0043008 ATP-dependent protein binding
Molecular Function GO:0046982 protein heterodimerization activity
Molecular Function GO:0044388 small protein activating enzyme binding
Molecular Function GO:0019948 SUMO activating enzyme activity
Biological Process GO:0033235 positive regulation of protein sumoylation
Biological Process GO:0016925 protein sumoylation

Reference

[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.