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Overview

Uniprot IDI3L7E5
Protein NameDNA replication licensing factor MCM3
Gene NameMCM3
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
152 VHYCPATKKTIERRY
248 YRCLPGKKGGYTSGT
314 IHGHDYVKKAILCLL
435 QGRVTIAKAGIHARL
556 DNLLHGTKKKKEKMV
576 KKYIHVAKIIKPALT
736 QETKEAQKVELSESR

Function

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division

Protein Sequence

10 MAGTVVLEDV 20 ELREAQRDYL 30 DFLDDEEDQG 40 IYQSKVRELI 50 SDNQYRLIVN 60 VNDLRRKNEK 70 RANRLLNNAF 80 EELVAFQRAL 90 KDFVASIDAT 100 YAKQYEEFYI 110 GLEGSFGSKH 120 VSPRTLTSCF 130 LSCVVCVEGI 140 VTKCSLVRPK 150 VVRSVHYCPA 160 TKKTIERRYT 170 DLTTLEAFPS 180 SSVYPTKDEE 190 NNPLETEYGL 200 SVYKDHQIIT 210 IQEMPEKAPA 220 GQLPRSVDVI 230 LDDDLVDKVK 240 PGDRVQVVGT 250 YRCLPGKKGG 260 YTSGTFRTVL 270 IACNVKQMSK 280 DAQPSFSAED 290 IAKIKKFSKT 300 RSKDIFDQLA 310 RSLAPSIHGH 320 DYVKKAILCL 330 LLGGVERDLE 340 NGSHIRGDIN 350 ILLIGDPSVA 360 KSQLLRYVLC 370 TAPRAIPTTG 380 RGSSGVGLTA 390 AVTTDQETGE 400 RRLEAGAMVL 410 ADRGVVCIDE 420 FDKMSDMDRT 430 AIHEVMEQGR 440 VTIAKAGIHA 450 RLNARCSVLA 460 AANPVYGRYD 470 QYKTPMENIG 480 LQDSLLSRFD 490 LLFIMLDQMD 500 PEQDREISDH 510 VLRMHRYRAP 520 GEQDGDAMPL 530 GSAVDILATD 540 DPNFSQEDQQ 550 DTQIYEKHDN 560 LLHGTKKKKE 570 KMVSAAFMKK 580 YIHVAKIIKP 590 ALTQESAAYI 600 AEEYSRLRSQ 610 DSMSSDTART 620 SPVTARTLET 630 LIRLATAHAK 640 ARMSKTVDLQ 650 DAEEAVELVQ 660 YAYFKKVLEK 670 EKKRKKRNED 680 DSETEDEGEK 690 SQEDQEQKRK 700 RRKTRHSDAK 710 DGDSYDPYDF 720 SDTEEEMPQV 730 HTPKAPDSQE 740 TKEAQKVELS 750 ESRLKAFKAA 760 LLEVFQEAHA 770 QSVGMNRLTE 780 SINRDKEEPF 790 SSSEIQAALS 800 RMQDDNQVMV SEGIVFLI

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005694 chromosome
Cellular Component GO:0042555 MCM complex
Cellular Component GO:0005634 nucleus
Cellular Component GO:0048471 perinuclear region of cytoplasm
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0003678 DNA helicase activity
Molecular Function GO:0003697 single-stranded DNA binding
Biological Process GO:0006271 DNA strand elongation involved in DNA replication
Biological Process GO:0000727 double-strand break repair via break-induced replication
Biological Process GO:1902975 mitotic DNA replication initiation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.