Overview
| Uniprot ID | I3LAE8 |
|---|
| Protein Name | PAT complex subunit CCDC47 |
|---|
| Gene Name | CCDC47 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 286 |
LSEFCSDKPKSGAKY |
Function
Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes. The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions. Within the MPT complex, the PAT subcomplex sequesters any highly polar regions in the transmembrane domains away from the non-polar membrane environment until they can be buried in the interior of the fully assembled protein. Within the PAT subcomplex, CCDC47 occludes the lateral gate of the SEC61 complex. Involved in the regulation of calcium ion homeostasis in the ER. Required for proper protein degradation via the ERAD (ER-associated degradation) pathway. Has an essential role in the maintenance of ER organization during embryogenesis
Protein Sequence
10
MKALHAFCVV
20
LLIFGSFSEA
30
KFDDFEDEED
40
IVEYDDNDFA
50
EFEDVPEDSV
60
TESPQRVITT
70
EDDEDETTVE
80
LEGQDENQEG
90
DFEDADTQEG
100
DTESEPYDDE
110
EFEGYEDKPD
120
TSSSKSKDPI
130
TIVDVPAHLQ
140
NSWESYYLEI
150
LMVTGLLAYI
160
MNYIIGKNKN
170
SRLAQAWFNT
180
HRELLESNFT
190
LVGDDGTNKE
200
ATSTGKLNQE
210
NEHIYNLWCS
220
GRVCCEGMLI
230
QLRFLKRQDL
240
LNVLARMMRP
250
VSDQVQIKVT
260
MNDEDMDTYV
270
FAVGTRKALV
280
RLQKEMQDLS
290
EFCSDKPKSG
300
AKYGLPDSLA
310
ILSEMGEVTE
320
GMMDTKMVHF
330
LTHYADKIES
340
VHFSDQFSGP
350
KIMQEEGQPL
360
KLPDTKRTLL
370
FTFNVPGSGN
380
TYPKDMEALL
390
PLMNMVIYSI
400
DKAKKFRLNR
410
EGKQKADKNR
420
ARVEENFLKL
430
THVQRQEAAQ
440
SRREEKKRAE
450
KERIMNEEDP
460
EKQRRLEEAA
470
LRREQKKLEK
480
KQMKMKQIKV
KAM
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0160064 |
multi-pass translocon complex |
| Cellular Component |
GO:0030867 |
rough endoplasmic reticulum membrane |
| Molecular Function |
GO:0005509 |
calcium ion binding |
| Molecular Function |
GO:0044183 |
protein folding chaperone |
| Molecular Function |
GO:0043022 |
ribosome binding |
| Biological Process |
GO:0032469 |
endoplasmic reticulum calcium ion homeostasis |
| Biological Process |
GO:0036503 |
ERAD pathway |
| Biological Process |
GO:0160063 |
multi-pass transmembrane protein insertion into ER membrane |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.