Search Results

Overview

Uniprot IDI3LEF8
Protein NamePeroxisomal multifunctional enzyme type 2
Gene NameHSD17B4
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
184 SLAVEGKKNNIHCNT
284 CDFDNATKPQRIQDS
81 DSVEAGEKIVKAALD
84 EAGEKIVKAALDAFG

Function

Bifunctional enzyme acting on the peroxisomal fatty acid beta-oxidation pathway. Catalyzes two of the four reactions in fatty acid degradation: hydration of 2-enoyl-CoA (trans-2-enoyl-CoA) to produce (3R)-3-hydroxyacyl-CoA, and dehydrogenation of (3R)-3-hydroxyacyl-CoA to produce 3-ketoacyl-CoA (3-oxoacyl-CoA), which is further metabolized by SCPx. Can use straight-chain and branched-chain fatty acids, as well as bile acid intermediates as substrates

Protein Sequence

10 MASMLNFYGR 20 VVLVTGAGGG 30 LGRTYALAFA 40 ERGASVVVND 50 LGGDMKGVGK 60 GSLAADKVVE 70 EIRRKGGKAV 80 ANYDSVEAGE 90 KIVKAALDAF 100 GRIDVVVNNA 110 GILRDRSFSR 120 ISDEDWDMIQ 130 RVHLRGSFMV 140 TRAAWDHMKK 150 QNFGRIIMTS 160 SAAGIYGNFG 170 QANYSAAKLG 180 LLGLSNSLAV 190 EGKKNNIHCN 200 TVAPVAGSRM 210 TQGFLPEDLI 220 EALKPEYVAP 230 LVLWLCHESC 240 EENGSVFEVG 250 AGWIGKLRWE 260 RTLGALVRQK 270 NQPMTPEAVK 280 ANWTKICDFD 290 NATKPQRIQD 300 SVSTVIEALS 310 KIDSSDGGIS 320 ANNLSHATSA 330 APSGLVEAVG 340 YKFPPFSSSY 350 TEVDTIMYAL 360 GVGASIKEPK 370 DLKFIYEGNS 380 DFSCLPTFGV 390 ILAQKSLGGG 400 GLAEIPGLSV 410 NFTKVLHGEH 420 YLELYKPLPN 430 AGDLKCEAVV 440 ADVLDKRSGL 450 VILIDVYSYS 460 GKELICYNQF 470 SVFVMGSGGF 480 GGKRTSDKDK 490 VAVAIPSRPP 500 DAILTDTTSL 510 NQAALYRLSG 520 DWNPLHIDPD 530 FASLAGFDRP 540 ILHGLCTFGF 550 SARHVLQQYA 560 DRDVLRFKAI 570 KVRFAKPVYP 580 GQTLQTEMWK 590 EGNRIHFQTK 600 VQETGDTVIS 610 NAYVDLVPTS 620 DTLAKIPSEG 630 GDLQSNLVFE 640 EIGRRLQDIG 650 QEMVKKVNAV 660 FEWHITKGEK 670 IAAKWTIDLK 680 NGAGKVYQGP 690 AKGSADATFI 700 LSDEVFMEVV 710 LGKLDPQKAF 720 FSGRLKARGN 730 IMLSQKLQMI LKDYAKI

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005782 peroxisomal matrix
Cellular Component GO:0005777 peroxisome
Molecular Function GO:0080023 (2E)-enoyl-CoA hydratase activity
Molecular Function GO:0106386 (3R)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity
Molecular Function GO:0003857 (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity
Molecular Function GO:0033989 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity
Molecular Function GO:0004300 enoyl-CoA hydratase activity
Molecular Function GO:0004303 estradiol 17-beta-dehydrogenase [NAD(P)+] activity
Molecular Function GO:0016853 isomerase activity
Molecular Function GO:0042803 protein homodimerization activity
Biological Process GO:0008209 androgen metabolic process
Biological Process GO:0008210 estrogen metabolic process
Biological Process GO:0006635 fatty acid beta-oxidation
Biological Process GO:0033540 fatty acid beta-oxidation using acyl-CoA oxidase
Biological Process GO:0006633 fatty acid biosynthetic process
Biological Process GO:0036112 medium-chain fatty-acyl-CoA metabolic process
Biological Process GO:0036111 very long-chain fatty-acyl-CoA metabolic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.