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Overview

Uniprot ID	I3LFX7
Protein Name	Squalene synthase
Gene Name	FDFT1
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
45	SSLKTCYKYLNQTSR

Function

Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway

Protein Sequence

10 MEFVKCLGHP 20 EEFYNLLRFQ 30 MGGRRKVIPK 40 MNQDSLSSSL 50 KTCYKYLNQT 60 SRSFAAVIQA 70 LDGEMRHAVC 80 IFYLVLRALD 90 TLEDDMTISI 100 EKKIPLLHNF 110 HLHLYESDWR 120 FTESKEKDRQ 130 VLEDFPTIAL 140 EFRNLAEKYQ 150 VVIVDVCRKM 160 GFGMAEFLNK 170 HVTSEREWDK 180 YCHYVAGLVG 190 IGLSRLFSAS 200 ELEDPLVGED 210 TERANSMGLF 220 LQKTNIIRDY 230 LEDQQEGREF 240 WPQEVWSKYV 250 KKLGDFAKPE 260 NVDLAVQCLN 270 ELVTSTLHHI 280 PDVITYLSRL 290 RNQSIFNFCA 300 IPQVMAIATL 310 AACYNNQQVF 320 KGVVKIRKGQ 330 AVTLMMDATN 340 MPAVKAIIHQ 350 YMEEVSWLSV 360 HGTVSLPCVS 370 LLQPHQTVWT 380 LLALRCGWWS 390 PVGPRCLFPG 400 QAVGSQARMT 410 SGLITGASLG YYYFCLL

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005789	endoplasmic reticulum membrane
Molecular Function	GO:0055056	D-glucose transmembrane transporter activity
Molecular Function	GO:0046872	metal ion binding
Molecular Function	GO:0051996	squalene synthase [NAD(P)H] activity
Biological Process	GO:0006695	cholesterol biosynthetic process
Biological Process	GO:0045338	farnesyl diphosphate metabolic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.