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Overview

Uniprot IDI3LHM3
Protein NameDNA topoisomerase I
Gene NameTOP1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
152 DDADYKPKKIKTEDI
166 IKKEKKRKLEEEEDG
227 KWKFLEHKGPVFAPP
284 IFRKNFFKDWRKEMT
395 INCSKDAKVPSPPPG
447 KGEKDWQKYETARRL
544 DSIRYYNKVPVEKRV
716 ATDREENKQIALGTS
739 ITVAWCKKWGVPIEK
750 PIEKIYNKTQREKFA

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone

Protein Sequence

10 MSGDHLHTDS 20 QIEADFRLND 30 SHKHKDKHKD 40 REHRHKEHKK 50 DKEKDREKSK 60 HSNSEHKDSE 70 KKHKEKEKTK 80 HKDGSSEKHK 90 DKHKDRDKEK 100 RKEEKIRASG 110 DAKIKKEKEN 120 GFSSPPRIKD 130 EPEDDGYFAP 140 PKEDIKPLKR 150 PRDEDDADYK 160 PKKIKTEDIK 170 KEKKRKLEEE 180 EDGKLKKTKN 190 KDKDKDKKVP 200 EPDSKKKKPK 210 KEEEQKWKWW 220 EEERYPEGIK 230 WKFLEHKGPV 240 FAPPYEPLPE 250 SVKFYYDGKV 260 MKLSPKAEEV 270 ATFFAKMLDH 280 EYTTKEIFRK 290 NFFKDWRKEM 300 TNEEKNIITN 310 LSKCDFTQMS 320 QYFKAQSEAR 330 KQMSKEEKLK 340 IKEENEKLLK 350 EYGFCIMDNH 360 RERIANFKIE 370 PPGLFRGRGN 380 HPKMGMLKRR 390 IMPEDIIINC 400 SKDAKVPSPP 410 PGHKWKEVRH 420 DNKVTWLVSW 430 TENIQGSIKY 440 IMLNPSSRIK 450 GEKDWQKYET 460 ARRLKKCVDK 470 IRNQYREDWK 480 SKEMKVRQRA 490 VALYFIDKLA 500 LRAGNEKEEG 510 ETADTVGCCS 520 LRVEHINLHP 530 ELDGQEYVVE 540 FDFLGKDSIR 550 YYNKVPVEKR 560 VFKNLQLFME 570 NKQPEDDLFD 580 RLNTGILNKH 590 LQDLMEGLTA 600 KVFRTYNASI 610 TLQQQLKELT 620 APDENIPAKI 630 LSYNRANRAV 640 AILCNHQRAP 650 PKTFEKSMMN 660 LQTKIDAKKE 670 QLADARRDLK 680 SAKADAKVLK 690 DAKTKKVVES 700 KKKAVQRLEE 710 QLMKLEVQAT 720 DREENKQIAL 730 GTSKLNYLDP 740 RITVAWCKKW 750 GVPIEKIYNK 760 TQREKFAWAI DMADEDYEF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005694 chromosome
Cellular Component GO:0005730 nucleolus
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity
Biological Process GO:0007059 chromosome segregation
Biological Process GO:0006260 DNA replication
Biological Process GO:0006265 DNA topological change
Biological Process GO:0048511 rhythmic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.