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Overview

Uniprot IDI3LK24
Protein NameAP-2 complex subunit alpha
Gene NameAP2A1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
117 RLINNAIKNDLASRN
217 LITCLCKKNPDDFKT
26 DIRNCKSKEAEIKRI
277 PPEDAAVKGRLVECL
296 NKAQEPPKSKKVQHS
35 AEIKRINKELANIRS
952 RLTLRTSKEPVSRHL

Function

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif

Protein Sequence

10 MPAVSKGDGM 20 RGLAVFISDI 30 RNCKSKEAEI 40 KRINKELANI 50 RSKFKGDKAL 60 DGYSKKKYVC 70 KLLFIFLLGH 80 DIDFGHMEAV 90 NLLSSNKYTE 100 KQIGYLFISV 110 LVNSNSELIR 120 LINNAIKNDL 130 ASRNPTFMCL 140 ALHCIANVGS 150 REMGEAFAAD 160 IPRILVAGDS 170 MDSVKQSAAL 180 CLLRLYKASP 190 DLVPMGEWTA 200 RVVHLLNDQH 210 MGVVTAAVSL 220 ITCLCKKNPD 230 DFKTCISLAV 240 SRLSRIVSSA 250 STDLQDYTYY 260 FVPAPWLSVK 270 LLRLLQCYPP 280 PEDAAVKGRL 290 VECLETVLNK 300 AQEPPKSKKV 310 QHSNAKNAIL 320 FETISLIIHY 330 DSEPNLLVRA 340 CNQLGQFLQH 350 RETNLRYLAL 360 ESMCTLASSE 370 FSHEAVKTHI 380 DTVINALKTE 390 RDVSVRQRAA 400 DLLYAMCDRS 410 NAKQIVSEML 420 RYLETADYAI 430 REEIVSLKVA 440 ILAEKYAVDY 450 SWYVDTILNL 460 IRIAGDYVSE 470 EVWYRVLQIV 480 TNRDDVQGYA 490 AKTVFEALQA 500 PACHENMVKV 510 GGYILGEFGN 520 LIAGDPRSSP 530 PVQFSLLHSK 540 FHLCSVATRA 550 LLLSTYIKFI 560 NLFPETKATI 570 QGVLRAGSQL 580 RNADVELQQR 590 AVEYLTLSSV 600 ASTDVLATVL 610 EEMPPFPERE 620 SSILAKLKRK 630 KGPGAGSALD 640 DGRRDPSSHD 650 INGGVEPTPS 660 TVSTPSPSAD 670 LLGLRAAPPP 680 AAPPAPSGAG 690 QLLVDVFSDG 700 PSDFGALGRI 710 WAYGFWDSVP 720 SGPPPCPPAP 730 SHLPPLPPAA 740 QPPPPPPRFV 750 CRNNGVLFEN 760 QLLQIGVKSE 770 FRQNLGRMYL 780 FYGNKTSVQF 790 QNFSPTVVHP 800 GDLQAHILLV 810 WPGSLVDGGA 820 QVQQVLNIEC 830 LRDFLTPPLL 840 SVRFRYGGAP 850 QSLTLKLPVT 860 INKFFQPTEM 870 AAQDFFQRWK 880 QLSLPQQEAQ 890 KIFKANHPMD 900 AEVTKAKLLG 910 FGSALLDNVD 920 PNPENFVGAG 930 IIQTKALQVG 940 CLLRLEPNAQ 950 AQMYRLTLRT 960 SKEPVSRHLC ELLAQQF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0030122 AP-2 adaptor complex
Molecular Function GO:0035615 clathrin adaptor activity
Biological Process GO:0072583 clathrin-dependent endocytosis
Biological Process GO:0006886 intracellular protein transport

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.