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Overview

Uniprot IDI3LKK0
Protein NameIsoleucine--tRNA ligase, cytoplasmic
Gene NameIARS1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
1010 ATIKAPLKPYPVPTS
1019 YPVPTSDKILIQEKM
1081 DNRLDLLKLKSVVTS
132 MRYSTEWKSTITRLG
169 VFKQLYDKGLVYRGV
285 RFPGACLKGKKYKPL
288 GACLKGKKYKPLFDY
298 PLFDYFVKCKENGAF
375 QYVKDADKNIIRTLK
382 KNIIRTLKEQGRLLI
606 QKMSKRKKNYPDPLS
844 DRKTIPIKYPLKEIV
878 IEELNVRKVTLSTDK
885 KVTLSTDKNKYGIHL

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA

Protein Sequence

10 MVQQVPENIS 20 FPAEEEKILQ 30 FWSDFNCFQE 40 CLKQSKHKPK 50 FTFYDGPPFA 60 TGLPHYGHIL 70 AGTIKDIVTR 80 YAHQSGFHVD 90 RRFGWDCHGL 100 PVEYEIDKTL 110 GIRGPEDVAK 120 LGIAEYNSQC 130 RAIVMRYSTE 140 WKSTITRLGR 150 WIDFDNDYKT 160 LYPQFMESVW 170 WVFKQLYDKG 180 LVYRGVKVMP 190 FSTACNTPLS 200 NFESHQNYKD 210 VQDPSIFVTF 220 PLEEDENISL 230 VAWTTTPWTL 240 PSNLAVCVNP 250 DLQYVKIKDV 260 VRGTLHILME 270 ARLSALYKLE 280 SDYEILERFP 290 GACLKGKKYK 300 PLFDYFVKCK 310 ENGAFTVLVD 320 NYVKEEEGTG 330 VVHQAPYFGA 340 DDYRVCVDFN 350 IIQKDSPPVC 360 PVDASGCFTE 370 EVTDFAGQYV 380 KDADKNIIRT 390 LKEQGRLLIA 400 STFTHSYPFC 410 WRSDTPLIYK 420 AVPSWFVRVE 430 HMVEQLLGNN 440 DLCYWVPEFV 450 REKRFGNWLK 460 DARDWAISRN 470 RYWGTPIPLW 480 VSDDFEEVVC 490 IGSVAELEEL 500 SGAKVSDLHR 510 ESIDHLTIPS 520 RCGKGLLHRI 530 SEVFDCWFES 540 GSMPYAQIHY 550 PFENKREFED 560 SFPADFIAEG 570 IDQTRGWFYT 580 LLVLATALFG 590 RPPFKNVIVN 600 GLVLASDGQK 610 MSKRKKNYPD 620 PLSIIHKYGA 630 DALRLYLINS 640 PVVRAENLRF 650 KEEGVRDVLK 660 DVLLPWYNAY 670 RFFIQNVLRL 680 QKEEEMEFLY 690 NENLVKESAN 700 ITDRWVLSFM 710 QSLIGFFETE 720 MAAYRLYTVV 730 PRLVKFVDVL 740 TNWYVRMNRR 750 RLKGENGVED 760 CVMALETLFS 770 VLLSLCRLMA 780 PYTPFLTELM 790 YQNLKMLIDP 800 VSVRDKDTLS 810 IHYLMLPHVR 820 EELIDKKTES 830 AVSRMQSVIE 840 LGRVIRDRKT 850 IPIKYPLKEI 860 VVIHQDPEAL 870 DEIKSLEKYI 880 IEELNVRKVT 890 LSTDKNKYGI 900 HLRAEPDHMV 910 LGKRLKGAFK 920 MVMTSIKQLS 930 SEELEWFQES 940 GTIVVEGHEL 950 HEEDIRLMYT 960 FDQAAGGTTQ 970 FEAHSDAQCN 980 LVPTDEITVY 990 YKAKSEGEYL 1000 NNVIKSHTEF 1010 IFATIKAPLK 1020 PYPVPTSDKI 1030 LIQEKMQLKG 1040 SDLEVTLTRG 1050 SSMPAPACAY 1060 VNLNICTNGS 1070 EQGGVLLLEN 1080 PKGDNRLDLL 1090 KLKSVVTSIF 1100 EVKNMELSVF 1110 HGETEIKNQT 1120 NLMSLSGKTL 1130 CVTSGSAPSV 1140 IDSPSTLLCQ 1150 YINLQLLNAD 1160 PQGCLMGTVG 1170 TLLLENPLGQ 1180 NGLTHQGLLY 1190 EAAKVFGLRS 1200 RKLKLFLDET 1210 QTEEITEETP 1220 MKTLNTKTVY 1230 VAVLPTTADL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
Cellular Component GO:0005829 cytosol
Molecular Function GO:0002161 aminoacyl-tRNA deacylase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004822 isoleucine-tRNA ligase activity
Molecular Function GO:0000049 tRNA binding
Biological Process GO:0006428 isoleucyl-tRNA aminoacylation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.