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Overview

Uniprot IDI3LLT2
Protein NameMultifunctional protein CAD
Gene NameCAD
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
186 LALDCGLKYNQIRCL

Function

Multifunctional protein that encodes the first 3 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5), aspartate transcarbamylase (ATCase; EC 2.1.3.2) and dihydroorotase (DHOase; EC 3.5.2.3). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate. In the last step, DHOase catalyzes the cyclization of carbamoyl aspartate to dihydroorotate

Protein Sequence

10 MAALVLEDGS 20 VLRGQPFGAA 30 VSTAGEVVFQ 40 TGMVGYPEAL 50 TDPSYKAQIL 60 VLTYPLIGNY 70 GIPPDEVDEF 80 GLSKWFESSG 90 IHVAGLVVGE 100 CCPTPSHWSA 110 ACTLHEWLQQ 120 HGIPGLQGVD 130 TRELTKKLRE 140 QGSLLGKLVQ 150 DGTEPSTLPF 160 LDPNARPLVP 170 EVSVKVPRVF 180 NAGGAPRILA 190 LDCGLKYNQI 200 RCLCRRGAEV 210 TVVPWDHALD 220 SQAYEGLFLS 230 NGPGDPASYP 240 SVVSALSRVL 250 SEPNPRPVFG 260 ICLGHQLLAL 270 AIGAKTYKMR 280 YGNRGHNQPC 290 LLVGSGRCFL 300 TSQNHGFAVE 310 TDSLPASWLP 320 LFTNANDRSN 330 EGIVHDSLPF 340 FSVQFHPEHQ 350 AGPSDMELLF 360 DIFLETVKEA 370 TAGNPGGQTV 380 RERLAERLCL 390 PGLPTPGSGL 400 PPPRKVLILG 410 SGGLSIGQAG 420 EFDYSGSQAI 430 KALKEENIQT 440 LLINPNIATV 450 QTSQGLADKV 460 YFLPITPHYV 470 TQVIRNERPD 480 GVLLTFGGQT 490 ALNCGVELTS 500 GVLARYGVRV 510 LGTPVETIEL 520 TEDRRAFASR 530 MAEIGEHVAP 540 SEAASSLEQA 550 QAAAERLGYP 560 VLVRAAFALG 570 GLGSGFASNK 580 EELSALVAPA 590 FAHTSQVLVD 600 KSLKGWKEIE 610 YEVVRDAYGN 620 CVTVCNMENL 630 DPLGIHTGES 640 IVVAPSQTLN 650 DREYQLLRQT 660 AIKVTQHLGI 670 VGECNVQYAL 680 NPESEQYYNE 690 VNARLSRSSA 700 LASKATGYPL 710 AYVAAKLALG 720 IPLPELRNSV 730 TGGTAAFEPS 740 LDYCVVKIPR 750 WDLSKFLRVS 760 TKIGSCMKSV 770 GEVMGIGRSF 780 EEAFQKALRM 790 VDENCVGFDH 800 TVKPVSDMEL 810 ETPTDKRIFV 820 VAAALWAGYS 830 VDRLYELTRI 840 DRWFLHRMKR 850 IIAHTQLLEQ 860 HRGQPLPPDL 870 LQQAKRLGFS 880 DKQIALAVLS 890 TELAVRKLRQ 900 ELGICPAVKQ 910 IDTVAAEWPA 920 QTNYLYLTYW 930 GTTHDLTFRT 940 PHVLVLGSGV 950 YRIGSSVEFD 960 WCAVGCIRQL 970 RKMGYKTIMV 980 NYNPETVSTD 990 YDMCDRLYFD 1000 EISFEVVMDI 1010 YELESPEGVI 1020 LSMGGQLPNN 1030 MAMALHRQQC 1040 RVLGTSPEAI 1050 DSAENRFKFS 1060 RLLDTIGISQ 1070 PQWRELSDLE 1080 SARQFCQTVG 1090 YPCVVRPSYV 1100 LSGAAMNVAY 1110 TDGDLERFLS 1120 SAAAVSKEHP 1130 VVISKFIQEA 1140 KEIDVDAVAC 1150 DGVVAAIAIS 1160 EHVENAGVHS 1170 GDATLVTPPQ 1180 DITAKTLERI 1190 KAIVHAVGQE 1200 LQVTGPFNLQ 1210 LIAKDDQLKV 1220 IECNVRVSRS 1230 FPFVSKTLGV 1240 DLVALATRVI 1250 MGEEVEPVGL 1260 MTGSGVVGVK 1270 VPQFSFSRLA 1280 GADVVLGVEM 1290 TSTGEVAGFG 1300 ESRCEAYLKA 1310 MLSTGFKIPK 1320 KNILLTIGSY 1330 KNKSELLPTV 1340 RLLESLGYSL 1350 YASLGTADFY 1360 TEHGVKVTAV 1370 DWHFEEAVDG 1380 ECPPQRSILE 1390 QLAEKNFELV 1400 INLSMRGAGG 1410 RRLSSFVTKG 1420 YRTRRLAADF 1430 SVPLIIDIKC 1440 TKLFVEALGQ 1450 IGPAPPLKVH 1460 VDCMTSQKLV 1470 RLPGLIDVHV 1480 HLREPGGTHK 1490 EDFASGTAAA 1500 LAGGVTMVCA 1510 MPNTRPPVID 1520 APALALAQKL 1530 AEAGARCDYT 1540 LFLGASSDNA 1550 GTLGPVAASA 1560 AGLKLYLNET 1570 FSELRLDSVA 1580 QWMEHFETWP 1590 SHLPIVAHAE 1600 RQSVAAILMV 1610 AQLTQRSVHI 1620 CHVARKEEIL 1630 LIKAAKARGL 1640 PVTCEVAPHH 1650 LFLSRDDLER 1660 LGPGKGEVRP 1670 ELGSRQDVDA 1680 LWENMAIIDC 1690 FASDHAPHTV 1700 EEKCGPRPPP 1710 GFPGLETMLP 1720 LLLTAVSEGR 1730 LSLDDLLQRL 1740 HHNPRRIFHL 1750 PPQEDTYVEV 1760 DLEHEWTVPS 1770 HMPFSKAHWT 1780 PFEGQKLKGT 1790 VRRVVLRGEV 1800 AYIDGQVLVP 1810 PGYGQDVRKW 1820 PQGAVPQLTP 1830 AAPATSEITT 1840 TPERPRRGIP 1850 VLPDGRFHLP 1860 PRIHRASDPG 1870 LPAEEPKEKS 1880 SRKAAEPDLM 1890 GTLDGTCYLP 1900 PPVPRQASPQ 1910 NLGAPGLLHP 1920 QTSPLLHSLV 1930 GQHILSVRQF 1940 TKDQMSHLFN 1950 VAHMLRMMVQ 1960 KERSLDILKG 1970 KVMASMFYEV 1980 STRTSSSFAA 1990 AMARLGGAVL 2000 SFSEATSSVQ 2010 KGESLADSVQ 2020 TMSCYADVVV 2030 LRHPQPGAVE 2040 LAAKHCRRPV 2050 INAGDGVGEH 2060 PTQALLDIFT 2070 IREELGTVNG 2080 MTITMVGDLK 2090 HGRTVHSLAC 2100 LLTQYRVSLR 2110 YVAPPSLRMP 2120 PDVRAFVASR 2130 GTKQEEFESI 2140 EEALPDTDVL 2150 YMTRIQKERF 2160 GSAQEYEACF 2170 GQFILTPHIM 2180 TRAKKKMVVM 2190 HPMPRVNEIS 2200 VEVDSDPRAA 2210 YFRQAENGMY 2220 IRMALLATVL GRF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0016363 nuclear matrix
Molecular Function GO:0016597 amino acid binding
Molecular Function GO:0004070 aspartate carbamoyltransferase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004087 carbamoyl-phosphate synthase (ammonia) activity
Molecular Function GO:0004088 carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Function GO:0004151 dihydroorotase activity
Molecular Function GO:0019899 enzyme binding
Molecular Function GO:0004359 glutaminase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process
Biological Process GO:0044205 'de novo' UMP biosynthetic process
Biological Process GO:0006520 amino acid metabolic process
Biological Process GO:0006541 glutamine metabolic process
Biological Process GO:0006225 UDP biosynthetic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.