Search Results
Overview
| Uniprot ID | I3LP02 |
|---|---|
| Protein Name | Acetyl-CoA acetyltransferase, mitochondrial |
| Gene Name | ACAT1 |
| Organism | Sus scrofa |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 222 | INSYTRSKAAWEAGK |
| 229 | KAAWEAGKFRSEVIP |
| 267 | FNKVPKLKTVFQKEN |
| 306 | AAKRLNVKPLARIAA |
| 337 | YAVPKVLKDAGLKKE |
Function
This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism
Protein Sequence
10
MAVLSVLVRG
20
ACGRSLLLRR
30
RMQEIRYVER
40
SYVSKPTLNE
50
VVIVSATRTP
60
IGSFLGSLSS
70
LPATKLGSIA
80
IQGAIEKAGI
90
PKEEVKEVYM
100
GNVLQGGEGQ
110
APTRQAVLGA
120
GLPISTPCTT
130
INKVCASGMK
140
AIMMASQNLM
150
CGHQDVMVAG
160
GMESMSNVPY
170
VMDRGTTPYG
180
GVKLEDLIVK
190
DGLTDVYNKI
200
HMGNCAENTA
210
KKLNIPRDEQ
220
DTYAINSYTR
230
SKAAWEAGKF
240
RSEVIPVTIS
250
VKGKPDTVVK
260
EDEEYKRVDF
270
NKVPKLKTVF
280
QKENGTVTAA
290
NASTLNDGAA
300
AVVLMTADAA
310
KRLNVKPLAR
320
IAAFADAAVD
330
PIDFPIAPAY
340
AVPKVLKDAG
350
LKKEDVTMWE
360
VNEAFSVVVL
370
ANIKMLEIDP
380
QKVNINGGAV
390
SLGHPIGMSG
400
ARIVVHLAHA
410
LKQGEYGLAS
420
ICNGGGGASA
MLIQKL
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0005783 | endoplasmic reticulum |
| Cellular Component | GO:0005739 | mitochondrion |
| Molecular Function | GO:0003985 | acetyl-CoA C-acetyltransferase activity |
| Molecular Function | GO:0034736 | cholesterol O-acyltransferase activity |
| Molecular Function | GO:0030955 | potassium ion binding |
| Biological Process | GO:0006085 | acetyl-CoA biosynthetic process |
| Biological Process | GO:0046356 | acetyl-CoA catabolic process |
| Biological Process | GO:0015937 | coenzyme A biosynthetic process |
| Biological Process | GO:0046952 | ketone body catabolic process |
| Biological Process | GO:0006550 | L-isoleucine catabolic process |
| Biological Process | GO:1902860 | propionyl-CoA biosynthetic process |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.