Search Results

Overview

Uniprot IDI3LSJ4
Protein NameSplicing factor 3B subunit 3
Gene NameSF3B3
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
102 QFLAVDPKGRAVMIS
12 EIVVSRGKILELLRP
51 FRLTGGTKDYIVVGS
513 DKRVNEWKTPGKKTI
522 PGKKTIVKCAVNQRQ
85 IHQETFGKSGCRRIV
941 RVLIGVGKLLRVYDL
950 LRVYDLGKKKLLRKC
956 GKKKLLRKCENKHIA
960 LLRKCENKHIANYIS

Function

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B3 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs

Protein Sequence

10 TKQQEIVVSR 20 GKILELLRPD 30 PNTGKVHTLL 40 TVEVFGVIRS 50 LMAFRLTGGT 60 KDYIVVGSDS 70 GRIVILEYQP 80 SKNMFEKIHQ 90 ETFGKSGCRR 100 IVPGQFLAVD 110 PKGRAVMISA 120 IEKQKLVYIL 130 NRDAAARLTI 140 SSPLEAHKAN 150 TLVYHVVGVD 160 VGFENPMFAC 170 LEMDYEEADN 180 DPTGEAAANT 190 QQTLTFYELD 200 LGLNHVVRKY 210 SEPLEEHGNF 220 LITVPGGSDG 230 PSGVLICSEN 240 YITYKNFGDQ 250 PDIRCPIPRR 260 RNDLDDPERG 270 MIFVCSATHK 280 TKSMFFFLAQ 290 TEQGDIFKIT 300 LETDEDMVTE 310 IRLKYFDTVP 320 VAAAMCVLKT 330 GFLFVASEFG 340 NHYLYQIAHL 350 GDDDEEPEFS 360 SAMPLEEGDT 370 FFFQPRPLKN 380 LVLVDELDSL 390 SPILFCQIAD 400 LANEDTPQLY 410 VACGRGPRSS 420 LRVLRHGLEV 430 SEMAVSELPG 440 NPNAVWTVRR 450 HIEDEFDAYI 460 IVSFVNATLV 470 LSIGETVEEV 480 TDSGFLGTTP 490 TLSCSLLGDD 500 ALVQVYPDGI 510 RHIRADKRVN 520 EWKTPGKKTI 530 VKCAVNQRQV 540 VIALTGGELV 550 YFEMDPSGQL 560 NEYTERKEMS 570 ADVVCMSLAN 580 VPPGEQRSRF 590 LAVGLVDNTV 600 RIISLDPSDC 610 LQPLSMQALP 620 AQPESLCIVE 630 MGGTEKQDEL 640 GERGSIGFLY 650 LNIGLQNGVL 660 LRTVLDPVTG 670 DLSDTRTRYL 680 GSRPVKLFRV 690 RMQGQEAVLA 700 MSSRSWLSYS 710 YQSRFHLTPL 720 SYETLEFASG 730 FASEQCPEGI 740 VAISTNTLRI 750 LALEKLGAVF 760 NQVAFPLQYT 770 PRKFVIHPES 780 NNLIIIETDH 790 NAYTEATKAQ 800 RKQQMAEEMV 810 EAAGEDEREL 820 AAEMAAAFLN 830 ENLPESIFGA 840 PKAGNGQWAS 850 VIRVMNPIQG 860 NTLDLVQLEQ 870 NEAAFSVAVC 880 RFSNTGDDWY 890 VLVGVAKDLI 900 LNPRSVAGGF 910 VYTYKLVNNG 920 EKLEFLHKTP 930 VEEVPAAIAP 940 FQGRVLIGVG 950 KLLRVYDLGK 960 KKLLRKCENK 970 HIANYISGIQ 980 TIGHRVIVSD 990 VQESFIWVRY 1000 KRNENQLIIF 1010 ADDTYPRWVT 1020 TASLLDYDTV 1030 AGADKFGNIC 1040 VVRLPPNTND 1050 EVDEDPTGNK 1060 ALWDRGLLNG 1070 ASQKAEVIMN 1080 YHVGETVLSL 1090 QKTTLIPGGS 1100 ESLVYTTLSG 1110 GIGILVPFTS 1120 HEDHDFFQHV 1130 EMHLRSEHPP 1140 LCGRDHLSFR 1150 SYYFPVKNVI 1160 DGDLCEQFNS 1170 MEPNKQKNVS 1180 EELDRTPPEV 1190 SKKLEDIRTR YAF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0071013 catalytic step 2 spliceosome
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005689 U12-type spliceosomal complex
Cellular Component GO:0005686 U2 snRNP
Cellular Component GO:0071005 U2-type precatalytic spliceosome
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0030620 U2 snRNA binding
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0042177 negative regulation of protein catabolic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.