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Overview

Uniprot IDI3LVK3
Protein NameLeucine--tRNA ligase, cytoplasmic
Gene NameLARS1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
1019 FASEAEDKVREDCCP
1028 REDCCPGKPLNVFRT
107 PIKACADKLKREIEL
1105 RRVPVLGKEHTEKTP
141 KTEDIILKDKAKGKK
274 EYTLIKLKVLEPYPC
282 VLEPYPCKLSGLKGK
544 DYGEEKWKEQTCQCL
552 EQTCQCLKNLETFCE
937 HFETSSGKLPDNKVI
942 SGKLPDNKVIASELG
955 LGNLPELKKYMKKVM
974 MIKENLEKTGPRVLD

Function

Aminoacyl-tRNA synthetase that catalyzes the specific attachment of leucine to its cognate tRNA (tRNA(Leu)). It performs tRNA aminoacylation in a two-step reaction: Leu is initially activated by ATP to form a leucyl-adenylate (Leu-AMP) intermediate; then the leucyl moiety is transferred to the acceptor 3' end of the tRNA to yield leucyl-tRNA. To improve the fidelity of catalytic reactions, it is also able to hydrolyze misactivated aminoacyl-adenylate intermediates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing)

Protein Sequence

10 MTKPPLERKG 20 TTKVDFLKKI 30 EKEIQQKWDT 40 EKVFEVNASD 50 LEKQTSKDKY 60 FVTFPYPYMN 70 GRLHLGHTFS 80 LSKCEFAVGY 90 QRLKGKTCLY 100 PFGLHCTGMP 110 IKACADKLKR 120 EIELYGCPPD 130 FPDEEEEEEE 140 INAKTEDIIL 150 KDKAKGKKSK 160 ASAKTGSSKY 170 QWDIMKSLGL 180 SDEEIVKFSE 190 AEHWLEYFPP 200 LAIQDLKRMG 210 LKVDWRRSFI 220 TTDVNPYYDS 230 FVRWQFLTLR 240 ERNKIKFGKR 250 YTIYSPKDGQ 260 PCMDHDRQTG 270 EGVGPQEYTL 280 IKLKVLEPYP 290 CKLSGLKGKN 300 VFLVAATLRP 310 ETMFGQTNCW 320 VHPEIKYIGF 330 ETVNGDIFIC 340 TPRAARNMSY 350 QGFTKDNGVV 360 PVVKELMGEE 370 ILGASLSAPL 380 TSYKVIYVLP 390 MLTIKEDKGT 400 GVVTSVPSDS 410 PDDFAAFRDL 420 KKKQALRAKY 430 GIRDDMVLPF 440 EPVPIIEIPG 450 FGKLSAVTLC 460 DELKVQSQND 470 REKLAEAKEK 480 LYLKGFYDGI 490 MLVDGFEGQK 500 VQDVKKSIRN 510 RMIDTGDAYA 520 YMEPEKQVMS 530 RSSDECVVAL 540 CDQWYLDYGE 550 EKWKEQTCQC 560 LKNLETFCEE 570 TRRNFEATLD 580 WLQEHACSRT 590 YGLGTRLPWD 600 EQWLIESLSD 610 STIYMAFYTA 620 AHLLQGGDIR 630 GQAESPLGIR 640 PQQMTKEVWD 650 YIFFKEAPFP 660 KTQIPKEKLD 670 QLKQEFEFWY 680 PVDLRVSGKD 690 LIPNHLSYFL 700 YNHVAMWPEE 710 SDKWPVAVRA 720 NGHLLLNSEK 730 MSKSTGNFLT 740 LTEALDKYSA 750 DGMRLALADA 760 GDTVEDANFV 770 EAMADAGVLR 780 LYTWVEWVKE 790 MVANWDSLRS 800 GPANTFNDRV 810 FAAKDKYREL 820 AIEGMHRDLV 830 FRFIEVQTLL 840 LAPFCPHLCE 850 HIWTLLGKPD 860 SIMNASWPVA 870 GPVDESLIRS 880 SQYLMEVAHD 890 LRLRLKNYMT 900 PAKGKKPDKQ 910 PPQKPSHCTI 920 YVAKDYPSWQ 930 HVTLSVLRSH 940 FETSSGKLPD 950 NKVIASELGN 960 LPELKKYMKK 970 VMPFVAMIKE 980 NLEKTGPRVL 990 DLQLEFDEQA 1000 VLMENIVYLT 1010 NSLELERIEV 1020 KFASEAEDKV 1030 REDCCPGKPL 1040 NVFRTEPGVL 1050 VSLVNPQPFS 1060 GHFSAKIEIR 1070 QGDNRDSVIR 1080 RLMKVDRGIK 1090 DLSKVKLMRF 1100 DDPLLGPRRV 1110 PVLGKEHTEK 1120 TPISEQAVFH 1130 VDLTSKKVHL 1140 TENGLRADIG DTMIYLVH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
Molecular Function GO:0002161 aminoacyl-tRNA deacylase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004823 leucine-tRNA ligase activity
Biological Process GO:0006429 leucyl-tRNA aminoacylation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.