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Overview

Uniprot IDK7GT57
Protein NameSpliceosome RNA helicase DDX39B
Gene NameDDX39B
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
173 ELAFQISKEYERFSK
198 FGGLSIKKDEEVLKK
204 KKDEEVLKKNCPHIV
205 KDEEVLKKNCPHIVV
230 RNKSLNLKHIKHFIL
233 SLNLKHIKHFILDEC
310 GLQQYYVKLKDNEKN
376 LSRYQQFKDFQRRIL
78 APAKKDVKGSYVSIH
95 GFRDFLLKPELLRAI

Function

Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly

Protein Sequence

10 MFHSLGKVES 20 VGMEEVAKAL 30 LMTDESSLWF 40 FSLALLSETS 50 ISFPLLFLEP 60 PLSPVLFCVN 70 PTAAGGDGAE 80 APAKKDVKGS 90 YVSIHSSGFR 100 DFLLKPELLR 110 AIVDCGFEHP 120 SEVQHECIPQ 130 AILGMDVLCQ 140 AKSGMGKTAV 150 FVLATLQQLE 160 PVTGQVSVLV 170 MCHTRELAFQ 180 ISKEYERFSK 190 YMPNVKVAVF 200 FGGLSIKKDE 210 EVLKKNCPHI 220 VVGTPGRILA 230 LARNKSLNLK 240 HIKHFILDEC 250 DKMLEQLDMR 260 RDVQEIFRMT 270 PHEKQVMMFS 280 ATLSKEIRPV 290 CRKFMQDPME 300 IFVDDETKLT 310 LHGLQQYYVK 320 LKDNEKNRKL 330 FDLLDVLEFN 340 QVVIFVKSVQ 350 RCIALAQLLV 360 EQNFPAIAIH 370 RGMPQEERLS 380 RYQQFKDFQR 390 RILVATNLFG 400 RGMDIERVNI 410 AFNYDMPEDS 420 DTYLHRVARA 430 GRFGTKGLAI 440 TFVSDENDAK 450 ILNDVQDRFE 460 VNISELPDEI 470 DISSYIEQTR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016607 nuclear speck
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016787 hydrolase activity
Molecular Function GO:0003676 nucleic acid binding
Molecular Function GO:0003724 RNA helicase activity
Biological Process GO:0006397 mRNA processing

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.