Search Results

Overview

Uniprot ID	M3TYT0
Protein Name	Rho-associated protein kinase 2
Gene Name	ROCK2
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
1173	KKFGWVKKYVIVSSK
1180	KYVIVSSKKILFYDS
1240	ANEGESKKEQEFPVE
605	NNRDLQDKNCLLETA
759	NLLLEAEKRCSILDC
802	KIEQETQKRCLTQND
884	KTQVRELKEECEEKT
895	EEKTKLCKELQQKKQ
901	CKELQQKKQELQDER

Function

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation

Protein Sequence

10 MSRPPPTGKM 20 PGAPEAVSGD 30 GAGASRQRKL 40 EALIRDPRSP 50 INVESLLDGL 60 NSLVLDLDFP 70 ALRKNKNIDN 80 FLNRYEKIVK 90 KIRGLQMKAE 100 DYDVVKVIGR 110 GAFGEVQLVR 120 HKASQKVYAM 130 KLLSKFEMIK 140 RSDSAFFWEE 150 RDIMAFANSP 160 WVVQLFCAFQ 170 DDKYLYMVME 180 YMPGGDLVNL 190 MSNYDVPEKW 200 AKFYTAEVVL 210 ALDAIHSMGL 220 IHRDVKPDNM 230 LLDKHGHLKL 240 ADFGTCMKMD 250 ETGMVHCDTA 260 VGTPDYISPE 270 VLKSQGGDGY 280 YGRECDWWSV 290 GVFLFEMLVG 300 DTPFYADSLV 310 GTYSKIMDHK 320 NSLCFPEDAE 330 ISKHAKNLIC 340 AFLTDREVRL 350 GRNGVEEIKQ 360 HPFFKNDQWN 370 WDNIRETAAP 380 VVPELSSDID 390 SSNFDDIEDD 400 KGDVETFPIP 410 KAFVGNQLPF 420 IGFTYYRENL 430 LLSDSPSCKE 440 NDSIQIRKNE 450 ESQEIQKKLY 460 TLEEHLSTEI 470 QAKEELEQKC 480 KSVNTRLEKV 490 AKELEEEIAL 500 RKNVESALRQ 510 LEREKALLQH 520 KNAEYQRKAD 530 HEADKKRNLE 540 NDVNSLKDQL 550 EDLKKRNQNS 560 QISTEKVNQL 570 QRQLDETNAL 580 LRTESDTAAR 590 LRKTQAESSK 600 QIQQLESNNR 610 DLQDKNCLLE 620 TAKLKLEKDF 630 INLQSVLESE 640 RRDRTHGSEI 650 INDLQGRISG 660 LEEDLKNGKI 670 LLTKVEMEKR 680 QLQERFTDLE 690 KEKNNMEIDM 700 TYQLKVIQQS 710 LEQEEAEHKA 720 TKARLADKNK 730 IYESIEEAKS 740 EAMKEMEKKL 750 LEERTLKQKV 760 ENLLLEAEKR 770 CSILDCDLKQ 780 SQQKINELLK 790 QKDVLNEDVR 800 NLTLKIEQET 810 QKRCLTQNDL 820 KMQTQQVNTL 830 KMSEKQLKQE 840 NNHLMEMKMS 850 LEKQNAELRK 860 ERQDADGQMK 870 ELQDQLEAEQ 880 YFSTLYKTQV 890 RELKEECEEK 900 TKLCKELQQK 910 KQELQDERDS 920 LAAQLEITLT 930 KADSEQLARS 940 IAEEQYSDLE 950 KEKIMKELEI 960 KEMMARHKQE 970 LTEKDTTIAS 980 LEETNRTLTS 990 DVANLANEKE 1000 ELNNKLKDAQ 1010 EQLSRLKDEE 1020 ISAAAIKAQF 1030 EKQLLTERTL 1040 KTQAVNKLAE 1050 IMNRKEPVKR 1060 GNDTDMRRKE 1070 KENRKLHMEL 1080 KSEREKLTQQ 1090 MIKYQKELNE 1100 MQAQIAEESQ 1110 IRIELQMTLD 1120 SKDSDIEQLR 1130 SQLQALHIGL 1140 DSSSIGSGPG 1150 DAEADDGFPE 1160 SRLEGWLSLP 1170 VRNNTKKFGW 1180 VKKYVIVSSK 1190 KILFYDSEQD 1200 KEQSNPYMVL 1210 DIDKLFHVRP 1220 VTQTDVYRAD 1230 AKEIPRIFQI 1240 LYANEGESKK 1250 EQEFPVEPVG 1260 EKSNCICHKG 1270 HEFIPTLYHF 1280 PTNCEACMKP 1290 LWHMFKPPPA 1300 LECRRCHIKC 1310 HKDHMDKKEE 1320 IIAPCKVYYD 1330 ISSAKNLLLL 1340 ANSTEEQQKW 1350 VSRLVKKIPK 1360 KPPAPDPFAR 1370 SSPRTSMKIQ 1380 QNQSIRRPSR QLAANKPS

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005813	centrosome
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0005886	plasma membrane
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0106310	protein serine kinase activity
Molecular Function	GO:0004674	protein serine/threonine kinase activity
Molecular Function	GO:0031267	small GTPase binding
Molecular Function	GO:0008270	zinc ion binding
Biological Process	GO:0030036	actin cytoskeleton organization
Biological Process	GO:0003180	aortic valve morphogenesis
Biological Process	GO:0071560	cellular response to transforming growth factor beta stimulus
Biological Process	GO:0070168	negative regulation of biomineral tissue development
Biological Process	GO:0010825	positive regulation of centrosome duplication
Biological Process	GO:1900182	positive regulation of protein localization to nucleus
Biological Process	GO:0032956	regulation of actin cytoskeleton organization
Biological Process	GO:0042752	regulation of circadian rhythm
Biological Process	GO:0007266	Rho protein signal transduction
Biological Process	GO:0048511	rhythmic process
Biological Process	GO:0006939	smooth muscle contraction

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.