Overview
| Uniprot ID | M3VH94 |
|---|
| Protein Name | Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 |
|---|
| Gene Name | PAPSS1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 171 |
VCEQRDVKGLYKKAR |
| 243 |
ELYVPENKLHLAKTD |
| 258 |
AETLPALKINKVDMQ |
Function
Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells
Protein Sequence
10
MEVPGSLCKK
20
VKLSNNAQNW
30
GMQRATNVTY
40
QAHHVSRNKR
50
GQVVGTRGGF
60
RGCTVWLTGL
70
SGAGKTTVSM
80
ALEEYLVCHG
90
IPCYTLDGDN
100
IRQGLNKNLG
110
FSPEDREENV
120
RRIAEVAKLF
130
ADAGLVCITS
140
FISPYTQDRN
150
NARQIHEGAS
160
LPFFEVFVDA
170
PLHVCEQRDV
180
KGLYKKARAG
190
EIKGFTGIDS
200
EYEKPEAPEL
210
VLKTDSCDVN
220
DCVQQVVELL
230
QERDIVPVDA
240
SYEVKELYVP
250
ENKLHLAKTD
260
AETLPALKIN
270
KVDMQWVQVL
280
AEGWATPLNG
290
FMREREYLQC
300
LHFDCLLDGG
310
VINLSVPIVL
320
TATQEDKERL
330
DGCTAFALMY
340
EGRRVAILRN
350
PEFFEHRKEE
360
RCARQWGTTC
370
KNHPYIKMVM
380
EQGDWLVGGD
390
LQVLDRIYWN
400
DGLDQYRFTP
410
TELKQKFKDM
420
NADAVFAFQL
430
RNPVHNGHAL
440
LMQDTHKQLL
450
DRGYRRPVLL
460
LHPLGGWTKD
470
DDVPLMWRMK
480
QHAAVLEEGV
490
LNPETTVVAI
500
FPSPMMYAGP
510
TEVQWHCRAR
520
MVAGANFYIV
530
GRDPAGMPHP
540
ETGKDLYEPT
550
HGAKVLTMAP
560
GLITLEIVPF
570
RVAAYNKKKK
580
RMDYYDSEHH
590
EDFEFISGTR
600
MRKLAREGQK
610
PPEGFMAPKA
620
WTVLMEYYKS
LEKA
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0004020 |
adenylylsulfate kinase activity |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0042803 |
protein homodimerization activity |
| Molecular Function |
GO:0004781 |
sulfate adenylyltransferase (ATP) activity |
| Biological Process |
GO:0050428 |
3'-phosphoadenosine 5'-phosphosulfate biosynthetic process |
| Biological Process |
GO:0000103 |
sulfate assimilation |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.