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Overview

Uniprot IDO00148
Protein NameATP-dependent RNA helicase DDX39A
Gene NameDDX39A
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
333 LSRYQQFKDFQRRIL
35 APPKKDIKGSYVSIH

Function

Helicase that plays an essential role in mRNA export and is involved in multiple steps in RNA metabolism including alternative splicing (PubMed:33941617, PubMed:38801080). Regulates nuclear mRNA export to the cytoplasm through association with ECD (PubMed:33941617). Also involved in spliceosomal uridine-rich small nuclear RNA (U snRNA) export by stimulating the RNA binding of adapter PHAX (PubMed:39011894). Plays a role in the negative regulation of type I IFN production by increasing the nuclear retention of antiviral transcripts and thus reducing their protein expression (PubMed:32393512). Independently of the interferon pathway, plays an antiviral role against alphaviruses by binding to a 5' conserved sequence element in the viral genomic RNA (PubMed:37949067)

Protein Sequence

10 MAEQDVENDL 20 LDYDEEEEPQ 30 APQESTPAPP 40 KKDIKGSYVS 50 IHSSGFRDFL 60 LKPELLRAIV 70 DCGFEHPSEV 80 QHECIPQAIL 90 GMDVLCQAKS 100 GMGKTAVFVL 110 ATLQQIEPVN 120 GQVTVLVMCH 130 TRELAFQISK 140 EYERFSKYMP 150 SVKVSVFFGG 160 LSIKKDEEVL 170 KKNCPHVVVG 180 TPGRILALVR 190 NRSFSLKNVK 200 HFVLDECDKM 210 LEQLDMRRDV 220 QEIFRLTPHE 230 KQCMMFSATL 240 SKDIRPVCRK 250 FMQDPMEVFV 260 DDETKLTLHG 270 LQQYYVKLKD 280 SEKNRKLFDL 290 LDVLEFNQVI 300 IFVKSVQRCM 310 ALAQLLVEQN 320 FPAIAIHRGM 330 AQEERLSRYQ 340 QFKDFQRRIL 350 VATNLFGRGM 360 DIERVNIVFN 370 YDMPEDSDTY 380 LHRVARAGRF 390 GTKGLAITFV 400 SDENDAKILN 410 DVQDRFEVNV 420 AELPEEIDIS TYIEQSR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0016020 membrane
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity
Biological Process GO:0006406 mRNA export from nucleus
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0045824 negative regulation of innate immune response
Biological Process GO:0046832 negative regulation of RNA export from nucleus
Biological Process GO:0006405 RNA export from nucleus

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[4] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.