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Overview

Uniprot IDO00299
Protein NameChloride intracellular channel protein 1
Gene NameCLIC1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
119 AKFSAYIKNSNPALN
13 PQVELFVKAGSDGAK
131 ALNDNLEKGLLKALK
135 NLEKGLLKALKVLDN
192 HIVQVVCKKYRGFTI
193 IVQVVCKKYRGFTIP
49 NVTTVDTKRRTETVQ
79 EVHTDTNKIEEFLEA

Function

In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Reduces selenite and dehydroascorbate and may act as an antioxidant during oxidative stress response (PubMed:25581026, PubMed:37759794). Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Involved in regulation of the cell cycle

Protein Sequence

10 MAEEQPQVEL 20 FVKAGSDGAK 30 IGNCPFSQRL 40 FMVLWLKGVT 50 FNVTTVDTKR 60 RTETVQKLCP 70 GGQLPFLLYG 80 TEVHTDTNKI 90 EEFLEAVLCP 100 PRYPKLAALN 110 PESNTAGLDI 120 FAKFSAYIKN 130 SNPALNDNLE 140 KGLLKALKVL 150 DNYLTSPLPE 160 EVDETSAEDE 170 GVSQRKFLDG 180 NELTLADCNL 190 LPKLHIVQVV 200 CKKYRGFTIP 210 EAFRGVHRYL 220 SNAYAREEFA 230 STCPDDEEIE 240 LAYEQVAKAL K

Gene Ontology

Classification GO ID Description
Cellular Component GO:0048471 perinuclear region of cytoplasm
Cellular Component GO:0072562 blood microparticle
Cellular Component GO:0005903 brush border
Cellular Component GO:0034707 chloride channel complex
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0016020 membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005635 nuclear envelope
Cellular Component GO:0031965 nuclear membrane
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0031982 vesicle
Molecular Function GO:0045296 cadherin binding
Molecular Function GO:0005254 chloride channel activity
Molecular Function GO:0045174 glutathione dehydrogenase (ascorbate) activity
Biological Process GO:0006821 chloride transport
Biological Process GO:0070527 platelet aggregation
Biological Process GO:0007165 signal transduction

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[5] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.