Search Results

Overview

Uniprot ID	O00429
Protein Name	Dynamin-1-like protein
Gene Name	DNM1L
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
597	RGMLKTSKAEELLAE

Function

Functions in mitochondrial and peroxisomal division (PubMed:11514614, PubMed:12499366, PubMed:17301055, PubMed:17460227, PubMed:17553808, PubMed:18695047, PubMed:18838687, PubMed:19342591, PubMed:19411255, PubMed:19638400, PubMed:23283981, PubMed:23530241, PubMed:23921378, PubMed:26992161, PubMed:27145208, PubMed:27145933, PubMed:27301544, PubMed:27328748, PubMed:29478834, PubMed:32439975, PubMed:32484300, PubMed:9570752, PubMed:9786947). Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism (PubMed:23530241, PubMed:23584531, PubMed:33850055). The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes (PubMed:23283981, PubMed:23921378, PubMed:29899447). While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane (PubMed:29899447). Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner (PubMed:32484300). Plays an important role in mitochondrial fission during mitosis (PubMed:19411255, PubMed:26992161, PubMed:27301544, PubMed:27328748). Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage (By similarity). Required for normal brain development, including that of cerebellum (PubMed:17460227, PubMed:26992161, PubMed:27145208, PubMed:27301544, PubMed:27328748). Facilitates developmentally regulated apoptosis during neural tube formation (By similarity). Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues (By similarity). Required for formation of endocytic vesicles (PubMed:20688057, PubMed:23792689, PubMed:9570752). Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles (PubMed:17015472, PubMed:23792689). Required for programmed necrosis execution (PubMed:22265414). Rhythmic control of its activity following phosphorylation at Ser-637 is essential for the circadian control of mitochondrial ATP production (PubMed:29478834)

Protein Sequence

10 MEALIPVINK 20 LQDVFNTVGA 30 DIIQLPQIVV 40 VGTQSSGKSS 50 VLESLVGRDL 60 LPRGTGIVTR 70 RPLILQLVHV 80 SQEDKRKTTG 90 EENGVEAEEW 100 GKFLHTKNKL 110 YTDFDEIRQE 120 IENETERISG 130 NNKGVSPEPI 140 HLKIFSPNVV 150 NLTLVDLPGM 160 TKVPVGDQPK 170 DIELQIRELI 180 LRFISNPNSI 190 ILAVTAANTD 200 MATSEALKIS 210 REVDPDGRRT 220 LAVITKLDLM 230 DAGTDAMDVL 240 MGRVIPVKLG 250 IIGVVNRSQL 260 DINNKKSVTD 270 SIRDEYAFLQ 280 KKYPSLANRN 290 GTKYLARTLN 300 RLLMHHIRDC 310 LPELKTRINV 320 LAAQYQSLLN 330 SYGEPVDDKS 340 ATLLQLITKF 350 ATEYCNTIEG 360 TAKYIETSEL 370 CGGARICYIF 380 HETFGRTLES 390 VDPLGGLNTI 400 DILTAIRNAT 410 GPRPALFVPE 420 VSFELLVKRQ 430 IKRLEEPSLR 440 CVELVHEEMQ 450 RIIQHCSNYS 460 TQELLRFPKL 470 HDAIVEVVTC 480 LLRKRLPVTN 490 EMVHNLVAIE 500 LAYINTKHPD 510 FADACGLMNN 520 NIEEQRRNRL 530 ARELPSAVSR 540 DKSSKVPSAL 550 APASQEPSPA 560 ASAEADGKLI 570 QDSRRETKNV 580 ASGGGGVGDG 590 VQEPTTGNWR 600 GMLKTSKAEE 610 LLAEEKSKPI 620 PIMPASPQKG 630 HAVNLLDVPV 640 PVARKLSARE 650 QRDCEVIERL 660 IKSYFLIVRK 670 NIQDSVPKAV 680 MHFLVNHVKD 690 TLQSELVGQL 700 YKSSLLDDLL 710 TESEDMAQRR 720 KEAADMLKAL 730 QGASQIIAEI RETHLW

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005903	brush border
Cellular Component	GO:0032991	protein-containing complex
Cellular Component	GO:0030672	synaptic vesicle membrane
Molecular Function	GO:0005525	GTP binding
Molecular Function	GO:0030742	GTP-dependent protein binding
Molecular Function	GO:0005096	GTPase activator activity
Molecular Function	GO:0003924	GTPase activity
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0008289	lipid binding
Molecular Function	GO:0008017	microtubule binding
Molecular Function	GO:0042803	protein homodimerization activity
Molecular Function	GO:0031267	small GTPase binding
Molecular Function	GO:0031625	ubiquitin protein ligase binding
Biological Process	GO:0006897	endocytosis
Biological Process	GO:0060047	heart contraction
Biological Process	GO:0048312	intracellular distribution of mitochondria
Biological Process	GO:0000266	mitochondrial fission
Biological Process	GO:0043653	mitochondrial fragmentation involved in apoptotic process
Biological Process	GO:0090149	mitochondrial membrane fission
Biological Process	GO:0007005	mitochondrion organization
Biological Process	GO:0016559	peroxisome fission
Biological Process	GO:0090141	positive regulation of mitochondrial fission
Biological Process	GO:0090023	positive regulation of neutrophil chemotaxis
Biological Process	GO:0050714	positive regulation of protein secretion
Biological Process	GO:0051259	protein complex oligomerization
Biological Process	GO:0070585	protein localization to mitochondrion
Biological Process	GO:1903578	regulation of ATP metabolic process
Biological Process	GO:1901524	regulation of mitophagy
Biological Process	GO:1900063	regulation of peroxisome organization
Biological Process	GO:0048511	rhythmic process
Cellular Component	GO:0005905	clathrin-coated pit
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005783	endoplasmic reticulum
Cellular Component	GO:0005789	endoplasmic reticulum membrane
Cellular Component	GO:0005794	Golgi apparatus
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005874	microtubule
Cellular Component	GO:0005741	mitochondrial outer membrane
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0048471	perinuclear region of cytoplasm
Cellular Component	GO:0005777	peroxisome

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[4] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.