Search Results

Overview

Uniprot ID	O00443
Protein Name	Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
Gene Name	PIK3C2A
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
613	AVNLPRSKTADVTSL

Function

Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. Involved in the regulation of ciliogenesis and trafficking of ciliary components (PubMed:31034465)

Protein Sequence

10 MAQISSNSGF 20 KECPSSHPEP 30 TRAKDVDKEE 40 ALQMEAEALA 50 KLQKDRQVTD 60 NQRGFELSSS 70 TRKKAQVYNK 80 QDYDLMVFPE 90 SDSQKRALDI 100 DVEKLTQAEL 110 EKLLLDDSFE 120 TKKTPVLPVT 130 PILSPSFSAQ 140 LYFRPTIQRG 150 QWPPGLPGPS 160 TYALPSIYPS 170 TYSKQAAFQN 180 GFNPRMPTFP 190 STEPIYLSLP 200 GQSPYFSYPL 210 TPATPFHPQG 220 SLPIYRPVVS 230 TDMAKLFDKI 240 ASTSEFLKNG 250 KARTDLEITD 260 SKVSNLQVSP 270 KSEDISKFDW 280 LDLDPLSKPK 290 VDNVEVLDHE 300 EEKNVSSLLA 310 KDPWDAVLLE 320 ERSTANCHLE 330 RKVNGKSLSV 340 ATVTRSQSLN 350 IRTTQLAKAQ 360 GHISQKDPNG 370 TSSLPTGSSL 380 LQEVEVQNEE 390 MAAFCRSITK 400 LKTKFPYTNH 410 RTNPGYLLSP 420 VTAQRNICGE 430 NASVKVSIDI 440 EGFQLPVTFT 450 CDVSSTVEII 460 IMQALCWVHD 470 DLNQVDVGSY 480 VLKVCGQEEV 490 LQNNHCLGSH 500 EHIQNCRKWD 510 TEIRLQLLTF 520 SAMCQNLART 530 AEDDETPVDL 540 NKHLYQIEKP 550 CKEAMTRHPV 560 EELLDSYHNQ 570 VELALQIENQ 580 HRAVDQVIKA 590 VRKICSALDG 600 VETLAITESV 610 KKLKRAVNLP 620 RSKTADVTSL 630 FGGEDTSRSS 640 TRGSLNPENP 650 VQVSINQLTA 660 AIYDLLRLHA 670 NSGRSPTDCA 680 QSSKSVKEAW 690 TTTEQLQFTI 700 FAAHGISSNW 710 VSNYEKYYLI 720 CSLSHNGKDL 730 FKPIQSKKVG 740 TYKNFFYLIK 750 WDELIIFPIQ 760 ISQLPLESVL 770 HLTLFGILNQ 780 SSGSSPDSNK 790 QRKGPEALGK 800 VSLPLFDFKR 810 FLTCGTKLLY 820 LWTSSHTNSV 830 PGTVTKKGYV 840 MERIVLQVDF 850 PSPAFDIIYT 860 TPQVDRSIIQ 870 QHNLETLEND 880 IKGKLLDILH 890 KDSSLGLSKE 900 DKAFLWEKRY 910 YCFKHPNCLP 920 KILASAPNWK 930 WVNLAKTYSL 940 LHQWPALYPL 950 IALELLDSKF 960 ADQEVRSLAV 970 TWIEAISDDE 980 LTDLLPQFVQ 990 ALKYEIYLNS 1000 SLVQFLLSRA 1010 LGNIQIAHNL 1020 YWLLKDALHD 1030 VQFSTRYEHV 1040 LGALLSVGGK 1050 RLREELLKQT 1060 KLVQLLGGVA 1070 EKVRQASGSA 1080 RQVVLQRSME 1090 RVQSFFQKNK 1100 CRLPLKPSLV 1110 AKELNIKSCS 1120 FFSSNAVPLK 1130 VTMVNADPMG 1140 EEINVMFKVG 1150 EDLRQDMLAL 1160 QMIKIMDKIW 1170 LKEGLDLRMV 1180 IFKCLSTGRD 1190 RGMVELVPAS 1200 DTLRKIQVEY 1210 GVTGSFKDKP 1220 LAEWLRKYNP 1230 SEEEYEKASE 1240 NFIYSCAGCC 1250 VATYVLGICD 1260 RHNDNIMLRS 1270 TGHMFHIDFG 1280 KFLGHAQMFG 1290 SFKRDRAPFV 1300 LTSDMAYVIN 1310 GGEKPTIRFQ 1320 LFVDLCCQAY 1330 NLIRKQTNLF 1340 LNLLSLMIPS 1350 GLPELTSIQD 1360 LKYVRDALQP 1370 QTTDAEATIF 1380 FTRLIESSLG 1390 SIATKFNFFI 1400 HNLAQLRFSG 1410 LPSNDEPILS 1420 FSPKTYSFRQ 1430 DGRIKEVSVF 1440 TYHKKYNPDK 1450 HYIYVVRILR 1460 EGQIEPSFVF 1470 RTFDEFQELH 1480 NKLSIIFPLW 1490 KLPGFPNRMV 1500 LGRTHIKDVA 1510 AKRKIELNSY 1520 LQSLMNASTD 1530 VAECDLVCTF 1540 FHPLLRDEKA 1550 EGIARSADAG 1560 SFSPTPGQIG 1570 GAVKLSISYR 1580 NGTLFIMVMH 1590 IKDLVTEDGA 1600 DPNPYVKTYL 1610 LPDNHKTSKR 1620 KTKISRKTRN 1630 PTFNEMLVYS 1640 GYSKETLRQR 1650 ELQLSVLSAE 1660 SLRENFFLGG 1670 VTLPLKDFNL 1680 SKETVKWYQL TAATYL

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0030136	clathrin-coated vesicle
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0005802	trans-Golgi network
Cellular Component	GO:0031982	vesicle
Molecular Function	GO:0016303	1-phosphatidylinositol-3-kinase activity
Molecular Function	GO:0046934	1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity
Molecular Function	GO:0035005	1-phosphatidylinositol-4-phosphate 3-kinase activity
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0030276	clathrin binding
Molecular Function	GO:0035091	phosphatidylinositol binding
Biological Process	GO:0016477	cell migration
Biological Process	GO:0048268	clathrin coat assembly
Biological Process	GO:0006897	endocytosis
Biological Process	GO:0007173	epidermal growth factor receptor signaling pathway
Biological Process	GO:0006887	exocytosis
Biological Process	GO:0008286	insulin receptor signaling pathway
Biological Process	GO:0061024	membrane organization
Biological Process	GO:0043491	phosphatidylinositol 3-kinase/protein kinase B signal transduction
Biological Process	GO:0006661	phosphatidylinositol biosynthetic process
Biological Process	GO:0036092	phosphatidylinositol-3-phosphate biosynthetic process
Biological Process	GO:0048015	phosphatidylinositol-mediated signaling
Biological Process	GO:0048008	platelet-derived growth factor receptor signaling pathway
Biological Process	GO:0010508	positive regulation of autophagy
Biological Process	GO:0090050	positive regulation of cell migration involved in sprouting angiogenesis
Biological Process	GO:0014829	vascular associated smooth muscle contraction

Reference

[1] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.