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Overview

Uniprot IDO09161
Protein NameCalsequestrin-2
Gene NameCasq2
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
40 RVVSLSEKNLKQMLK

Function

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, especially at the interface between subunits. Can bind around 60 Ca(2+) ions. Regulates the release of lumenal Ca(2+) via the calcium release channel RYR2; this plays an important role in triggering muscle contraction. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats

Protein Sequence

10 MKRIYLLMVG 20 VYLLSLSGAE 30 EGLNFPTYDG 40 KDRVVSLSEK 50 NLKQMLKRYD 60 LLCLYYHEPV 70 SSDKVSQKQF 80 QLKEIVLELV 90 AQVLEHKNIG 100 FVMVDSRKEA 110 KLAKRLGFSE 120 EGSLYVLKGD 130 RTIEFDGEFA 140 ADVLVEFLLD 150 LIEDPVEIVN 160 NKLEVQAFER 170 IEDQTKLLGF 180 FKNEDSEYYK 190 AFQEAAEHFQ 200 PYIKFFATFD 210 KAVAKKLSLK 220 MNEVGFYEPF 230 MDEPNVIPNK 240 PYTEEELVEF 250 VKEHQRPTLR 260 RLRPEDMFET 270 WEDDLNGIHI 280 VAFAEKSDPD 290 GYEFLEILKQ 300 VARDNTDNPD 310 LSILWIDPDD 320 FPLLVAYWEK 330 TFKIDLFKPQ 340 IGVVNVTDAD 350 SIWMEIPDDD 360 DLPTAEELED 370 WIEDVLSGKI 380 NTEDDDNEDE 390 DDDGDDNDDD 400 DDDDDDNDNS 410 DEDNEDSDDD DDDDE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0030314 junctional membrane complex
Cellular Component GO:0016529 sarcoplasmic reticulum
Cellular Component GO:0033018 sarcoplasmic reticulum lumen
Cellular Component GO:0030018 Z disc
Molecular Function GO:0005509 calcium ion binding
Molecular Function GO:0140314 calcium ion sequestering activity
Molecular Function GO:0048306 calcium-dependent protein binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0005080 protein kinase C binding
Biological Process GO:0060048 cardiac muscle contraction
Biological Process GO:0071313 cellular response to caffeine
Biological Process GO:0006874 intracellular calcium ion homeostasis
Biological Process GO:0043267 negative regulation of potassium ion transport
Biological Process GO:0051258 protein polymerization
Biological Process GO:0086004 regulation of cardiac muscle cell contraction
Biological Process GO:0010881 regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
Biological Process GO:0010649 regulation of cell communication by electrical coupling
Biological Process GO:0002027 regulation of heart rate
Biological Process GO:0060306 regulation of membrane repolarization
Biological Process GO:1905024 regulation of membrane repolarization during ventricular cardiac muscle cell action potential
Biological Process GO:0006937 regulation of muscle contraction
Biological Process GO:0010880 regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
Biological Process GO:0045214 sarcomere organization
Biological Process GO:0051208 sequestering of calcium ion

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.