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Overview

Uniprot IDO09165
Protein NameCalsequestrin-1
Gene NameCasq1
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
113 FGLVDSEKDAAVAKK
55 RVINVNAKNYKNVFK

Function

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions (By similarity). Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca(2+) entry (SOCE) activity (By similarity)

Protein Sequence

10 MRATDRMGAR 20 AVSELRLALL 30 FVLVLGTPRL 40 GVQGEDGLDF 50 PEYDGVDRVI 60 NVNAKNYKNV 70 FKKYEVLALL 80 YHEPPEDDKA 90 SQRQFEMEEL 100 ILELAAQVLE 110 DKGVGFGLVD 120 SEKDAAVAKK 130 LGLTEEDSVY 140 VFKGDEVIEY 150 DGEFSADTLV 160 EFLLDVLEDP 170 VELIEGEREL 180 QAFENIEDEI 190 KLIGYFKSKD 200 SEHYKAYEDA 210 AEEFHPYIPF 220 FATFDSKVAK 230 KLTLKLNEID 240 FYEAFMEEPM 250 TIPDKPNSEE 260 EIVSFVEEHR 270 RSTLRKLKPE 280 SMYETWEDDL 290 DGIHIVAFAE 300 EADPDGYEFL 310 ETLKAVAQDN 320 TENPDLSIIW 330 IDPDDFPLLV 340 PYWEKTFDID 350 LSAPQIGVVN 360 VTDADSIWME 370 MDNEEDLPSA 380 DELEDWLEDV 390 LEGEINTEDD 400 DDDDDDDDDD DDDDD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0014802 terminal cisterna
Cellular Component GO:0014804 terminal cisterna lumen
Cellular Component GO:0030018 Z disc
Molecular Function GO:0005509 calcium ion binding
Molecular Function GO:0042802 identical protein binding
Biological Process GO:0007029 endoplasmic reticulum organization
Biological Process GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
Biological Process GO:1901341 positive regulation of store-operated calcium channel activity
Biological Process GO:0051258 protein polymerization
Biological Process GO:0006937 regulation of muscle contraction
Biological Process GO:0010880 regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
Biological Process GO:0051282 regulation of sequestering of calcium ion
Biological Process GO:0014809 regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion
Biological Process GO:2001256 regulation of store-operated calcium entry
Biological Process GO:0014894 response to denervation involved in regulation of muscle adaptation
Biological Process GO:0009408 response to heat
Biological Process GO:0045214 sarcomere organization
Biological Process GO:0007519 skeletal muscle tissue development
Cellular Component GO:0031674 I band
Cellular Component GO:0014701 junctional sarcoplasmic reticulum membrane
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0030016 myofibril
Cellular Component GO:0042383 sarcolemma
Cellular Component GO:0016529 sarcoplasmic reticulum
Cellular Component GO:0033018 sarcoplasmic reticulum lumen
Cellular Component GO:0030315 T-tubule

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.