Search Results

Overview

Uniprot IDO14556
Protein NameGlyceraldehyde-3-phosphate dehydrogenase, testis-specific
Gene NameGAPDHS
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
128 DSTHGRYKGSVEFRN
151 EISVYQCKEPKQIPW
154 VYQCKEPKQIPWRAV
258 HSYTATQKTVDGPSR
287 PASTGAAKAVTKVIP
291 GAAKAVTKVIPELKG
297 TKVIPELKGKLTGMA
299 VIPELKGKLTGMAFR
331 PAPYSAIKEAVKAAA
97 VLRACMEKGVKVVAV

Function

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis (By similarity). May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity)

Protein Sequence

10 MSKRDIVLTN 20 VTVVQLLRQP 30 CPVTRAPPPP 40 EPKAEVEPQP 50 QPEPTPVREE 60 IKPPPPPLPP 70 HPATPPPKMV 80 SVARELTVGI 90 NGFGRIGRLV 100 LRACMEKGVK 110 VVAVNDPFID 120 PEYMVYMFKY 130 DSTHGRYKGS 140 VEFRNGQLVV 150 DNHEISVYQC 160 KEPKQIPWRA 170 VGSPYVVEST 180 GVYLSIQAAS 190 DHISAGAQRV 200 VISAPSPDAP 210 MFVMGVNEND 220 YNPGSMNIVS 230 NASCTTNCLA 240 PLAKVIHERF 250 GIVEGLMTTV 260 HSYTATQKTV 270 DGPSRKAWRD 280 GRGAHQNIIP 290 ASTGAAKAVT 300 KVIPELKGKL 310 TGMAFRVPTP 320 DVSVVDLTCR 330 LAQPAPYSAI 340 KEAVKAAAKG 350 PMAGILAYTE 360 DEVVSTDFLG 370 DTHSSIFDAK 380 AGIALNDNFV 390 KLISWYDNEY 400 GYSHRVVDLL RYMFSRDK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005829 cytosol
Molecular Function GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Molecular Function GO:0051287 NAD binding
Molecular Function GO:0050661 NADP binding
Biological Process GO:0030317 flagellated sperm motility
Biological Process GO:0006006 glucose metabolic process
Biological Process GO:0006096 glycolytic process
Biological Process GO:0045821 positive regulation of glycolytic process

Reference

[1] Yan M, Tu H, Tang S, Gai Z, Shi Q et al.. Lactylated Proteomic Analysis Reveals Functional Implications of Lysine Lactylation In Asthenozoospermia.. Mol Cell Proteomics 24(12):101439. 2025 Dec. PMID: 41192556.